EP 3221813 A1 20170927 - METHODS RELATING TO THE DETECTION OF RECURRENT AND NON-SPECIFIC DOUBLE STRAND BREAKS IN THE GENOME
Title (en)
METHODS RELATING TO THE DETECTION OF RECURRENT AND NON-SPECIFIC DOUBLE STRAND BREAKS IN THE GENOME
Title (de)
VERFAHREN IM ZUSAMMENHANG MIT DER ERKENNUNG VON REZIDIVIERENDEN UND NICHTSPEZIFISCHEN DOPPELSTRÄNGIGEN BRÜCHEN IM GENOM
Title (fr)
PROCÉDÉS RELATIFS À LA DÉTECTION DE BRIS BICATÉNAIRES RÉCURRENTS ET NON SPÉCIFIQUES DANS LE GÉNOME
Publication
Application
Priority
- US 201462082395 P 20141120
- US 2015061758 W 20151120
Abstract (en)
[origin: WO2016081798A1] Described herein are methods and systems relating to high throughput, genome-wide translocation sequencing (HTGTS) and/or detection of double-stranded DNA break (DSB) locations. The methods described herein can comprise generating DSBs in a nucleic acid sequence and performing nested PCR with the primers described herein. Described herein is an enhanced HTGTS approach. The assays and methods described herein permit the measurement of various DNA double-strand break (DSB) activities either intrinsic to the biological system or from outside agents.
IPC 8 full level
G06F 19/22 (2011.01); G16B 30/10 (2019.01)
CPC (source: EP US)
C12Q 1/6869 (2013.01 - EP US); G16B 30/00 (2019.01 - EP US); G16B 30/10 (2019.01 - EP US); C12Q 2523/303 (2013.01 - US); C12Q 2525/191 (2013.01 - US); C12Q 2535/122 (2013.01 - US); C12Q 2549/119 (2013.01 - US); C12Q 2563/131 (2013.01 - US)
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
WO 2016081798 A1 20160526; EP 3221813 A1 20170927; EP 3221813 A4 20180418; US 10640820 B2 20200505; US 2018346977 A1 20181206
DOCDB simple family (application)
US 2015061758 W 20151120; EP 15861815 A 20151120; US 201515527790 A 20151120