EP 3247789 A4 20180912 - MICROFLUIDICS BASED FETAL CELL DETECTION AND ISOLATION FOR NON-INVASIVE PRENATAL TESTING
Title (en)
MICROFLUIDICS BASED FETAL CELL DETECTION AND ISOLATION FOR NON-INVASIVE PRENATAL TESTING
Title (de)
AUF MIKROFLUIDIK BASIERENDE FÖTUSZELLENDETEKTION UND -ISOLIERUNG FÜR NICHTINVASIVE PRÄNATALE TESTS
Title (fr)
DÉTECTION DE CELLULES F TALES BASÉE SUR LA MICROFLUIDIQUE ET ISOLEMENT POUR DES TESTS PRÉNATAUX NON INVASIFS
Publication
Application
Priority
- US 201562107261 P 20150123
- US 2016013865 W 20160119
Abstract (en)
[origin: WO2016118484A1] Embodiments disclosed herein provides methods for isolation of fetal cells for non¬ invasive prenatal diagnosis, comprising: providing a maternal blood sample; applying the maternal blood sample to a filter integrated on a microfluidic device to thereby enrich the nucleated blood cells from the maternal blood sample; labeling the enriched nucleated blood cells, within the microfluidic device, with a fluorescent binding moiety or affinity molecule that specifically binds to a fetal cell-specific antigen or a non-fetal cell-specific antigen; and isolating the fetal cells. Embodiments disclosed herein provide integrated microfluidic devices for non-invasive isolation of fetal cells, comprising: a filter; a binding moiety or affinity molecule that specifically binds to a fetal cell-specific antigen or a non-fetal cell- specific antigen; and a microscopy-visualizable chamber.
IPC 8 full level
B01L 3/00 (2006.01); C12M 1/12 (2006.01); C12N 5/0775 (2010.01); C12Q 1/6804 (2018.01); C12Q 1/6883 (2018.01); G01N 33/53 (2006.01); G01N 33/569 (2006.01); G01N 33/80 (2006.01)
CPC (source: CN EP KR US)
B01L 3/502753 (2013.01 - CN EP KR US); C12N 5/0641 (2013.01 - EP US); C12Q 1/68 (2013.01 - CN); C12Q 1/6816 (2013.01 - KR); C12Q 1/6881 (2013.01 - EP US); C12Q 1/6883 (2013.01 - US); G01N 33/00 (2013.01 - CN); G01N 33/53 (2013.01 - KR); G01N 33/5302 (2013.01 - EP US); G01N 33/56966 (2013.01 - EP US); G01N 33/582 (2013.01 - KR); G01N 33/80 (2013.01 - EP US); B01L 2200/0647 (2013.01 - CN EP KR US); B01L 2200/0652 (2013.01 - KR); B01L 2200/0668 (2013.01 - US); B01L 2300/0681 (2013.01 - CN EP KR US); C12N 2501/599 (2013.01 - EP US); C12Q 2565/601 (2013.01 - KR); C12Q 2565/629 (2013.01 - KR); C12Q 2600/158 (2013.01 - US)
Citation (search report)
- [XI] US 2007059716 A1 20070315 - BALIS ULYSSES [US], et al
- [XI] US 2007059774 A1 20070315 - GRISHAM MICHAEL [US], et al
- [XI] US 2014030788 A1 20140130 - CHEN GRACE [US], et al
- [X] KR 20130061114 A 20130610 - MAXBIOTECH CO [KR]
- [A] US 2004142463 A1 20040722 - WALKER GEORGE [US], et al
- [A] US 5641628 A 19970624 - BIANCHI DIANA W [US]
- See references of WO 2016118484A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
WO 2016118484 A1 20160728; AU 2016209521 A1 20170817; CA 2974373 A1 20160728; CN 107206380 A 20170926; EP 3247789 A1 20171129; EP 3247789 A4 20180912; JP 2018508194 A 20180329; KR 20170120105 A 20171030; MX 2017009485 A 20171115; US 2018015470 A1 20180118
DOCDB simple family (application)
US 2016013865 W 20160119; AU 2016209521 A 20160119; CA 2974373 A 20160119; CN 201680006681 A 20160119; EP 16740575 A 20160119; JP 2017538577 A 20160119; KR 20177022338 A 20160119; MX 2017009485 A 20160119; US 201615545604 A 20160119