EP 3280818 A2 20180214 - METHODS FOR SELECTING ENZYMES HAVING LIPASE ACTIVITY
Title (en)
METHODS FOR SELECTING ENZYMES HAVING LIPASE ACTIVITY
Title (de)
VERFAHREN ZUR SELEKTION VON ENZYMEN MIT LIPASE-AKTIVITÄT
Title (fr)
PROCÉDÉS DE SÉLECTION D'ENZYMES PRÉSENTANT UNE ACTIVITÉ DE LIPASE
Publication
Application
Priority
- US 201562144001 P 20150407
- US 2016026469 W 20160407
Abstract (en)
[origin: WO2016164596A2] Provided herein are methods and means for enhancing lipase activity. The system makes use of an emulsion for in vitro compartmentalization of a library of synthetic compounds which have a polynucleotide linked to a lipase substrate (e.g., a triglyceride). Expressed polypeptides having greater lipase activity will preferentially hydrolyze the substrate from the linked polynucleotide. Genes encoding polypeptides having less lipase activity will remain linked to the substrate and may be removed to enrich the library for more active variants. Also described are synthetic compounds and emulsions which can be used in the methods.
IPC 8 full level
CPC (source: CN EP US)
C12N 9/20 (2013.01 - CN); C12Q 1/34 (2013.01 - CN EP US); C12Q 1/44 (2013.01 - US); C12Y 301/01003 (2013.01 - CN); G01N 2333/92 (2013.01 - CN)
Citation (search report)
See references of WO 2016164596A2
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
WO 2016164596 A2 20161013; WO 2016164596 A3 20161222; CN 107864658 A 20180330; EP 3280818 A2 20180214; US 2018355404 A1 20181213
DOCDB simple family (application)
US 2016026469 W 20160407; CN 201680032769 A 20160407; EP 16763997 A 20160407; US 201615563692 A 20160407