Global Patent Index - EP 3299472 A1

EP 3299472 A1 20180328 - METHOD FOR LABELING OLIGONUCLEOTIDE PROBES

Title (en)

METHOD FOR LABELING OLIGONUCLEOTIDE PROBES

Title (de)

VERFAHREN ZUR MARKIERUNG VON OLIGONUKLEOTIDSONDEN

Title (fr)

PROCEDE DE MARQUAGE D'OLIGONUCLEOTIDES

Publication

EP 3299472 A1 20180328 (EN)

Application

EP 16190862 A 20160927

Priority

EP 16190862 A 20160927

Abstract (en)

The present invention provides a novel method for labelling nucleic acid probes. The method uses a ligase catalysed reaction to connect a nucleic acid probe with pre-prepared nucleic acid label carrier molecules under the presence of a stabilizing complementary splint oligonucleotide. The method allows for an easy, cheap and fast labelling of multiple probes with multiple different labels. In this way, the costs and effort for the generation of single molecule Fluorescent In Situ Hybridization (smFISH) assays was significantly reduced. The invention further provides methods for the generation of FISH libraries and labelling kits comprising the novel tools of the invention.

IPC 8 full level

C12Q 1/68 (2018.01)

CPC (source: EP US)

C12Q 1/6841 (2013.01 - EP US); C12Q 2521/501 (2013.01 - US); C12Q 2525/101 (2013.01 - US); C12Q 2525/179 (2013.01 - US); C12Q 2525/191 (2013.01 - US); C12Q 2525/204 (2013.01 - US); C12Q 2533/107 (2013.01 - US)

C-Set (source: EP US)

C12Q 1/6841 + C12Q 2521/501 + C12Q 2525/179 + C12Q 2525/191 + C12Q 2533/107

Citation (applicant)

KRICKA ET AL., ANN CLIN BIOCHEM., vol. 39, 2002, pages 114 - 29

Citation (search report)

  • [A] WO 2012148477 A1 20121101 - CELLULAR RES INC [US], et al
  • [A] YU LIU ET AL: "Specific labeling: An effective tool to explore the RNA world", BIOESSAYS, vol. 38, no. 2, 14 December 2015 (2015-12-14), GB, pages 192 - 200, XP055354654, ISSN: 0265-9247, DOI: 10.1002/bies.201500119
  • [A] B. MORITZ ET AL: "Simple methods for the 3' biotinylation of RNA", RNA, vol. 20, no. 3, 21 January 2014 (2014-01-21), US, pages 421 - 427, XP055354650, ISSN: 1355-8382, DOI: 10.1261/rna.042986.113
  • [A] NILSEN TIMOTHY W: "Site-specific labeling of RNA", COLD SPRING HARBOR PROTOCOLS, COLD SPRING HARBOR LABORATORY PRESS, US, vol. 2013, no. 3, 1 March 2013 (2013-03-01), pages 269 - 275, XP009183741, ISSN: 1559-6095, DOI: 10.1101/PDB.PROT072918
  • [A] F. BEHNAM ET AL: "A Straightforward DOPE (Double Labeling of Oligonucleotide Probes)-FISH (Fluorescence In Situ Hybridization) Method for Simultaneous Multicolor Detection of Six Microbial Populations", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 78, no. 15, 11 May 2012 (2012-05-11), US, pages 5138 - 5142, XP055355298, ISSN: 0099-2240, DOI: 10.1128/AEM.00977-12

Designated contracting state (EPC)

AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

Designated extension state (EPC)

BA ME

DOCDB simple family (publication)

EP 3299472 A1 20180328; CN 110050072 A 20190723; CN 110050072 B 20240315; EP 3519588 A1 20190807; EP 3519588 B1 20201202; ES 2856763 T3 20210928; JP 2020501507 A 20200123; JP 7200092 B2 20230106; US 2019264269 A1 20190829; WO 2018060249 A1 20180405

DOCDB simple family (application)

EP 16190862 A 20160927; CN 201780057771 A 20170927; EP 17784203 A 20170927; EP 2017074505 W 20170927; ES 17784203 T 20170927; JP 2019512972 A 20170927; US 201716334708 A 20170927