EP 3305912 B1 20211006 - KIT FOR TOGETHER DETECTING MULTIPLE TARGET NUCLEIC ACIDS DIFFERING FROM EACH OTHER AND DETECTION METHOD USING THE SAME
Title (en)
KIT FOR TOGETHER DETECTING MULTIPLE TARGET NUCLEIC ACIDS DIFFERING FROM EACH OTHER AND DETECTION METHOD USING THE SAME
Title (de)
NACHWEISKIT ZUR GLEICHZEITIGEN DETEKTION MEHRERER SICH UNTERSCHEIDENDER ZIELNUKLEINSÄUREN UND NACHWEISVERFAHREN MIT VERWENDUNG DAVON
Title (fr)
KIT DE DÉTECTION SIMULTANÉE DE MULTIPLE ACIDES NUCLÉIQUES CIBLES DISTINCTS ENTRE EUX ET PROCÉDÉ DE DÉTECTION UTILISANT CELUI-CI
Publication
Application
Priority
- JP 2015113979 A 20150604
- JP 2016063831 W 20160510
Abstract (en)
[origin: EP3305912A1] Provided is a kit for detecting multiple target nucleic acids capable of simultaneously amplifying and detecting multiple genes by means of one reaction vessel containing one kind of reaction solution and one kind of labels. Solution may contain first target nucleic acid (10) and second target nucleic acid (20) each of which dissociates at denaturation temperature T0. The solution further contains: DNA polymerase (30); a first target's primer (13) at annealing temperature T1 bonding with first single strands derived from the first target nucleic acid; a second target's primer (23) at annealing temperature T1 bonding with second single strands derived from the second target nucleic acid; a first target's probe (15) at annealing temperature T1 bonding with the first single strands derived from the first target nucleic acid; and a second target's probe (25) at second target detection temperature T3 which is lower than the annealing temperature T1 and elongation temperature T2 bonding with the second single strands derived from the second target nucleic acid. A condition that: T0 is higher than T2; T2 is not lower than T1; and T1 is higher than T3 is satisfied.
IPC 8 full level
C12Q 1/68 (2018.01); C12N 15/09 (2006.01); C12Q 1/6816 (2018.01)
CPC (source: EP KR US)
C12Q 1/68 (2013.01 - US); C12Q 1/6816 (2013.01 - EP US); C12Q 1/686 (2013.01 - KR US); C12Q 1/689 (2013.01 - EP US); C12Q 2527/101 (2013.01 - KR); C12Q 2527/107 (2013.01 - KR); C12Q 2563/107 (2013.01 - KR)
Citation (examination)
EP 2116614 A1 20091111 - QIAGEN GMBH [DE], et al
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
EP 3305912 A1 20180411; EP 3305912 A4 20180411; EP 3305912 B1 20211006; AU 2016269875 A1 20171102; AU 2016269875 A2 20171116; AU 2016269875 B2 20190509; BR 112017026001 A2 20181106; CA 2983428 A1 20161208; CA 2983428 C 20211207; CN 107849617 A 20180327; CN 107849617 B 20211102; DK 3305912 T3 20211206; ES 2899826 T3 20220314; JP 6720161 B2 20200708; JP WO2016194552 A1 20180322; KR 102077577 B1 20200217; KR 20170139150 A 20171218; MX 2017015673 A 20180619; MY 182821 A 20210205; PH 12017502015 A1 20180402; SG 11201708920P A 20171129; TW 201708545 A 20170301; TW I686481 B 20200301; US 2018155764 A1 20180607; US 2021238653 A1 20210805; WO 2016194552 A1 20161208
DOCDB simple family (application)
EP 16802989 A 20160510; AU 2016269875 A 20160510; BR 112017026001 A 20160510; CA 2983428 A 20160510; CN 201680031912 A 20160510; DK 16802989 T 20160510; ES 16802989 T 20160510; JP 2016063831 W 20160510; JP 2017521758 A 20160510; KR 20177033935 A 20160510; MX 2017015673 A 20160510; MY PI2017704387 A 20160510; PH 12017502015 A 20171106; SG 11201708920P A 20160510; TW 105115749 A 20160520; US 201615576923 A 20160510; US 202117205364 A 20210318