EP 3307786 A4 20190116 - METHODS AND COMPOSITIONS FOR PRODUCING A CHIMERIC POLYPEPTIDE
Title (en)
METHODS AND COMPOSITIONS FOR PRODUCING A CHIMERIC POLYPEPTIDE
Title (de)
VERFAHREN UND ZUSAMMENSETZUNGEN ZUR HERSTELLUNG EINES CHIMÄREN PEPTIDS
Title (fr)
PROCÉDÉS ET COMPOSITIONS DE PRODUCTION D'UN POLYPEPTIDE CHIMÉRIQUE
Publication
Application
Priority
- US 201562174994 P 20150612
- US 201562220060 P 20150917
- US 2016036233 W 20160607
Abstract (en)
[origin: WO2016200822A1] The present invention provides methods and compositions for converting a first polypeptide into a chimeric polypeptide. The invention includes two vectors: a first vector including the sequence of the first polypeptide and a second vector including a second polypeptide. The vectors include complementary site-specific recombination motifs such that site-specific recombination between the two vectors results in the generation of a chimeric polypeptide including at least a portion of the first polypeptide and at least a portion of the second polypeptide. A site-specific recombination motif may be positioned within an intron or within a coding sequence on the first or second vector.
IPC 8 full level
C12N 15/10 (2006.01); C07K 16/00 (2006.01); C07K 19/00 (2006.01); C12N 15/13 (2006.01); C12N 15/62 (2006.01); C12N 15/85 (2006.01)
CPC (source: CN EP US)
C07K 16/00 (2013.01 - CN EP US); C12N 15/10 (2013.01 - CN EP US); C12N 15/1037 (2013.01 - CN EP US); C12N 15/63 (2013.01 - CN US); C07K 2317/35 (2013.01 - CN EP US); C07K 2317/51 (2013.01 - CN US); C07K 2317/515 (2013.01 - CN US); C07K 2317/62 (2013.01 - CN EP US); C07K 2317/622 (2013.01 - CN EP US); C07K 2317/64 (2013.01 - CN EP US); C07K 2319/00 (2013.01 - CN EP US)
Citation (search report)
- [A] WO 2015085079 A2 20150611 - AXIOMX INC [US]
- [A] WO 9319172 A1 19930930 - CAMBRIDGE ANTIBODY TECH [GB], et al
- [A] WO 0105961 A1 20010125 - CLONTECH LAB INC [US]
- [A] WO 9521914 A1 19950817 - PASTEUR MERIEUX SERUMS VACC [FR], et al
- [A] HOLLAND ERIKA G ET AL: "AXM mutagenesis: An efficient means for the production of libraries for directed evolution of proteins", JOURNAL OF IMMUNOLOGICAL METHODS, vol. 394, no. 1, 13 May 2013 (2013-05-13), pages 55 - 61, XP028672935, ISSN: 0022-1759, DOI: 10.1016/J.JIM.2013.05.003
- [A] DIANE L. BUHR ET AL: "Use of micro-emulsion technology for the directed evolution of antibodies", METHODS, vol. 58, no. 1, 1 September 2012 (2012-09-01), US, pages 28 - 33, XP055284719, ISSN: 1046-2023, DOI: 10.1016/j.ymeth.2012.07.007
- [A] MARGARET MACRIS KISS ET AL: "Phage ESCape: An emulsion-based approach for the selection of recombinant phage display antibodies", JOURNAL OF IMMUNOLOGICAL METHODS., vol. 367, no. 1-2, 1 March 2011 (2011-03-01), NL, pages 17 - 26, XP055284871, ISSN: 0022-1759, DOI: 10.1016/j.jim.2010.09.034
- [XP] BATONICK M ET AL: "pMINERVA: A donor-acceptor system for the in vivo recombineering of scFv into IgG molecules", JOURNAL OF IMMUNOLOGICAL METHODS, vol. 431, 3 February 2016 (2016-02-03), pages 22 - 30, XP029444485, ISSN: 0022-1759, DOI: 10.1016/J.JIM.2016.02.003
- See references of WO 2016200822A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
WO 2016200822 A1 20161215; WO 2016200822 A8 20170223; AU 2016274571 A1 20180104; AU 2016274571 B2 20220331; CA 2989143 A1 20161215; CN 107922508 A 20180417; CN 107922508 B 20210604; CN 113307864 A 20210827; EP 3307786 A1 20180418; EP 3307786 A4 20190116; EP 3307786 B1 20200805; EP 3786293 A1 20210303; US 11192939 B2 20211207; US 2016362476 A1 20161215; US 2018230198 A1 20180816; US 9969792 B2 20180515
DOCDB simple family (application)
US 2016036233 W 20160607; AU 2016274571 A 20160607; CA 2989143 A 20160607; CN 201680047211 A 20160607; CN 202110529712 A 20160607; EP 16808130 A 20160607; EP 20188612 A 20160607; US 201615175739 A 20160607; US 201815950782 A 20180411