Global Patent Index - EP 3307911 A4

EP 3307911 A4 20181031 - AMPLIFICATION ASSAY WITH A PROBE COMPETITOR

Title (en)

AMPLIFICATION ASSAY WITH A PROBE COMPETITOR

Title (de)

AMPLIFIKATIONSVERFAHREN MIT EINEM SONDENKONKURRENT

Title (fr)

ESSAI D'AMPLIFICATION AVEC UN COMPÉTITEUR DE SONDES

Publication

EP 3307911 A4 20181031 (EN)

Application

EP 16808529 A 20160613

Priority

  • US 201562174279 P 20150611
  • US 2016037259 W 20160613

Abstract (en)

[origin: WO2016201444A1] Methods and compositions for detecting an allelic form of a target. In an exemplary method, partitions may be created that collectively contain at least one first allelic form and a second allelic form of a target. Each partition may contain (i) a same probe capable of binding specifically to each of the first and second allelic forms of the target and (ii) a competitor configured to bind selectively to the second allelic form relative to the first allelic form and to block binding of the probe to the second allelic form. The first allelic form of the target may be amplified in the partitions. A signal may be detected from a label of the probe while the label is contained by the partitions. A number of partitions that are positive (or negative) for the at least one first allelic form may be determined based on the signal.

IPC 8 full level

C12Q 1/68 (2018.01); C12P 19/34 (2006.01)

CPC (source: EP US)

C12Q 1/6827 (2013.01 - EP US); G16B 30/00 (2019.01 - US); C12Q 2563/159 (2013.01 - EP US)

Citation (search report)

  • [I] WO 2012095639 A2 20120719 - GENEFIRST LTD [GB], et al
  • [I] YANWEI JIA ET AL: "Kinetic Hairpin Oligonucleotide Blockers for Selective Amplification of Rare Mutations", SCIENTIFIC REPORTS, vol. 4, 1 August 2014 (2014-08-01), XP055334804, DOI: 10.1038/srep05921
  • [Y] SUNDBERG S O ET AL: "Quasi-digital PCR: Enrichment and quantification of rare DNA variants", BIOMEDICAL MICRODEVICES SPRINGER USA, vol. 16, no. 4, August 2014 (2014-08-01), pages 639 - 644, XP002784871, ISSN: 1387-2176
  • [Y] WWW REPORTS: "Rare allele enrichment and detection by allele-specific PCR, competitive probe blocking, and melting analysis", BIOTECHNIQUES.COM, 1 January 2011 (2011-01-01), XP055328556, Retrieved from the Internet <URL:http://www.biotechniques.com/multimedia/archive/00159/BTN_A_000113668_O_159847a.pdf> [retrieved on 20161213], DOI: 10.2144/000113668
  • [Y] GINYA ET AL: "Development of the Handy Bio-Strand and its application to genotyping of OPRM1 (A118G)", ANALYTICAL BIOCHEMISTRY, ELSEVIER, AMSTERDAM, NL, vol. 367, no. 1, 21 June 2007 (2007-06-21), pages 79 - 86, XP022126376, ISSN: 0003-2697, DOI: 10.1016/J.AB.2007.04.052
  • See references of WO 2016201444A1

Designated contracting state (EPC)

AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

DOCDB simple family (publication)

WO 2016201444 A1 20161215; CN 107614701 A 20180119; CN 107614701 B 20210720; EP 3307911 A1 20180418; EP 3307911 A4 20181031; EP 3307911 B1 20201209; US 2016362731 A1 20161215; US 9868981 B2 20180116

DOCDB simple family (application)

US 2016037259 W 20160613; CN 201680031658 A 20160613; EP 16808529 A 20160613; US 201615181173 A 20160613