EP 3308167 A4 20190213 - USE OF FLUORESCENCE FOR THE QUICK AND EASY DETERMINATION OF S-ADENOSYLMETHIONINE, S-ADENOSYLHOMOCYSTEINE AND HOMOCYSTEINE
Title (en)
USE OF FLUORESCENCE FOR THE QUICK AND EASY DETERMINATION OF S-ADENOSYLMETHIONINE, S-ADENOSYLHOMOCYSTEINE AND HOMOCYSTEINE
Title (de)
VERWENDUNG VON FLUORESZENZ ZUR SCHNELLEN UND LEICHTEN BESTIMMUNG VON S-ADENOSYLMETHIONIN, S-ADENOSYLHOMOCYSTEIN HOMOCYSTEIN
Title (fr)
UTILISATION DE LA FLUORESCENCE POUR LA DÉTERMINATION RAPIDE ET FACILE DE LA S-ADÉNOSYLMÉTHIONINE, DE LA S-ADÉNOSYLHOMOCYSTÉINE ET DE L'HOMOCYSTÉINE
Publication
Application
Priority
- US 201562166044 P 20150525
- US 2016034202 W 20160525
Abstract (en)
[origin: CN108291908A] The invention provides immunochromatographic test strips and methods for detecting and quantifying SAM, S-Adenosylhomocysteine (SAH) and Homocysteine (HCy) in a sample, comprising: (a) making fluorophore conjugated antibodies; (b) immobilizing SAM, SAH and HCy on a solid support; (c) providing a sample, combining said sample with a conjugate selected from the group consisting of lanthanide chelateconjugates and quantum dot conjugates (QD) with anti-SAM, anti-SAH or anti-HCy, wherein said combining is performed under conditions that allow formation of a competitive complex comprising said conjugate, said SAM, SAH or HCy on the solid support and SAM, SAH or HCy in a sample when present; and (d) detecting the presence of the complex, if present, by monitoring a spectral emission mediated bythe fluorescent conjugates in the complex, wherein the emission indicates the presence and quantity of SAM, SAH or HCy in the sample.
IPC 8 full level
G01N 33/533 (2006.01); C07K 16/00 (2006.01); C12Q 1/48 (2006.01); G01N 33/543 (2006.01); G01N 33/573 (2006.01)
CPC (source: EP US)
G01N 33/533 (2013.01 - EP); G01N 33/54373 (2013.01 - EP US); C07K 2319/60 (2013.01 - EP); G01N 2333/4737 (2013.01 - EP US); G01N 2800/50 (2013.01 - EP)
Citation (search report)
- [XY] US 2009263879 A1 20091022 - CHANG CHIU CHIN [US], et al
- [Y] GASPARYAN V K: "Synthesis of Water-Soluble CdSe Quantum Dots With Various Fluorescent Properties and Their Application in Immunoassay for Determination of C-Reactive Protein", JOURNAL OF FLUORESCENCE, PLENUM PUBLISHING, NEW YORK, US, vol. 24, no. 5, 18 July 2014 (2014-07-18), pages 1433 - 1438, XP035388195, ISSN: 1053-0509, [retrieved on 20140718], DOI: 10.1007/S10895-014-1426-8
- [Y] TARKKINEN P ET AL: "ULTRARAPID, ULTRASENSITIVE ONE-STEP KINETIC IMMUNOASSAY FOR C-REACTIVE PROTEIN (CRP) IN WHOLE BLOOD SAMPLES: MEASUREMENT OF THE ENTIRE CRP CONCENTRATION RANGE WITH A SINGLE SAMPLE DILUTION", CLINICAL CHEMISTRY, AMERICAN ASSOCIATION FOR CLINICAL CHEMISTRY, WASHINGTON, DC, vol. 48, no. 2, 1 February 2002 (2002-02-01), pages 269 - 277, XP001115951, ISSN: 0009-9147
- See references of WO 2016191507A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
CN 108291908 A 20180717; EP 3308167 A1 20180418; EP 3308167 A4 20190213
DOCDB simple family (application)
CN 201680032653 A 20160525; EP 16800682 A 20160525