EP 3333571 B1 20210630 - METHOD AND KIT FOR PARALLEL QUANTIFICATION OF SPLICE VARIANTS OF VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF-A)
Title (en)
METHOD AND KIT FOR PARALLEL QUANTIFICATION OF SPLICE VARIANTS OF VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF-A)
Title (de)
VERFAHREN UND KIT ZUR GLEICHZEITIGEN QUANTIFIZIERUNG VON SPLEISSVARIANTEN DES VASKULÄREN ENDOTHELIALEN WACHSTUMSFAKTORS (VEGF-A)
Title (fr)
PROCÉDÉ DE QUANTIFICATION PARALLÈLE DE VARIANTES D'ÉPISSAGE DU FACTEUR DE CROISSANCE ENDOTHÉLIAL VASCULAIRE (VEGF-A)
Publication
Application
Priority
- JP 2015153656 A 20150803
- JP 2016071821 W 20160726
Abstract (en)
[origin: EP3333571A1] There are numerous proteins having different in vivo effects depending on variants. In order to gain a more accurate understanding of an in vivo effect of a protein, when the protein in a sample is quantified, it is necessary to measure an amount of each variant that can be present. Provided is a method for performing parallel quantification of variants of a protein having 2 or more variants using mass spectrometry. The method includes: protease-digesting a protein in a sample; detecting, using mass spectrometry, among peptides obtained by the protease digestion, peptides having amino acid sequences specific to the variants without separating the peptides; and determining amounts of the variants in the sample based on results of the detection.
IPC 8 full level
G01N 33/68 (2006.01)
CPC (source: EP US)
G01N 1/4044 (2013.01 - US); G01N 1/405 (2013.01 - US); G01N 33/483 (2013.01 - US); G01N 33/68 (2013.01 - US); G01N 33/6848 (2013.01 - EP US); G01N 2333/475 (2013.01 - US); G01N 2333/49 (2013.01 - EP); H01J 49/26 (2013.01 - EP)
Citation (examination)
STACY D. SHERROD ET AL: "Label-Free Quantitation of Protein Modifications by Pseudo Selected Reaction Monitoring with Internal Reference Peptides", JOURNAL OF PROTEOME RESEARCH, vol. 11, no. 6, 17 June 2012 (2012-06-17), pages 3467 - 3479, XP055662530, ISSN: 1535-3893, DOI: 10.1021/pr201240a
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
EP 3333571 A1 20180613; EP 3333571 A4 20190313; EP 3333571 B1 20210630; CN 107923874 A 20180417; CN 107923874 B 20201009; JP 6537614 B2 20190703; JP WO2017022562 A1 20180628; SG 11201800894S A 20180328; US 2018231563 A1 20180816; WO 2017022562 A1 20170209
DOCDB simple family (application)
EP 16832841 A 20160726; CN 201680045133 A 20160726; JP 2016071821 W 20160726; JP 2017532514 A 20160726; SG 11201800894S A 20160726; US 201615750063 A 20160726