Global Patent Index - EP 3351935 A1

EP 3351935 A1 20180725 - PROCESS FOR MULTI-ANALYSES OF RARE CELLS EXTRACTED OR ISOLATED FROM BIOLOGICAL SAMPLES THROUGH FILTRATION

Title (en)

PROCESS FOR MULTI-ANALYSES OF RARE CELLS EXTRACTED OR ISOLATED FROM BIOLOGICAL SAMPLES THROUGH FILTRATION

Title (de)

VERFAHREN ZUR MULTIANALYSE VON AUS BIOLOGISCHEN PROBEN MITTELS FILTRATION EXTRAHIERTEN ODER ISOLIERTEN SELTENEN ZELLEN

Title (fr)

PROCÉDÉ POUR MULTI-ANALYSES DE CELLULES RARES EXTRAITES DE OU ISOLÉES PAR FILTRATION À PARTIR D'ÉCHANTILLONS BIOLOGIQUES

Publication

EP 3351935 A1 20180725 (EN)

Application

EP 17210576 A 20130523

Priority

  • US 201261651437 P 20120524
  • EP 13724594 A 20130523
  • EP 2013060671 W 20130523

Abstract (en)

A process for isolating or extracting rare cells from a biological sample comprising filtering a biological sample, which may be treated or diluted, through a filter that has a pore size, pore density or other physical properties that retain rare cells, but which permits other kinds of cells to pass through the filter. This process also comprises multiple analyses performed on rare cells after their extraction or isolation by filtration to diagnostically identify the presence of rare cells in a biological sample and to use their diagnostic identification and molecular characterization for diagnostic purposes such as for early diagnosis of diseases, namely for early diagnosis of cancer and to select, guide, monitor treatments and in particular to select targeted treatments and to monitor the response and/or resistance to them. A kit comprising tools, equipment and/or reagents to accomplish both the filtration step and various kinds of multiple analyses performed after isolation and extraction of the rare cells by filtration.

IPC 8 full level

G01N 33/50 (2006.01); C12N 5/00 (2006.01)

CPC (source: CN EP RU US)

A61P 9/00 (2017.12 - EP); A61P 11/00 (2017.12 - EP); A61P 29/00 (2017.12 - EP); A61P 31/00 (2017.12 - EP); A61P 35/00 (2017.12 - EP); A61P 35/04 (2017.12 - EP); A61P 43/00 (2017.12 - EP); C12Q 1/6876 (2013.01 - US); C12Q 1/6886 (2013.01 - US); G01N 33/5091 (2013.01 - CN EP RU US); G01N 33/554 (2013.01 - US); G01N 33/57492 (2013.01 - EP); G01N 2800/00 (2013.01 - CN); G01N 2800/52 (2013.01 - CN); G01N 2800/7028 (2013.01 - CN EP)

Citation (applicant)

  • US 7651838 B2 20100126 - PATERLINI-BRECHOT PATRIZIA [FR]
  • US 2009226957 A1 20090910 - PATERLINI-BRECHOT PATRIZIA [FR]
  • IMUDIA AN; KUMAR S; DIAMOND MP; DECHERNEY AH; ARMANT DR: "Transcervical retrieval of fetal cells in the practice of modern medicine: a review of the current literature and future direction", FERTIL STERIL, vol. 93, 2010, pages 1725 - 30, XP055181093, DOI: doi:10.1016/j.fertnstert.2009.11.022
  • IMUDIA AN ET AL., FERTIL STERIL, vol. 93, 2010, pages 1725 - 30
  • SAKER ET AL., PRENATAL DIAGNOSIS, 2006
  • GOYA T; ASAMURA H; YOSHIMURA H ET AL.: "Prognosis of 6644 resected non-small cell lung cancers in Japan: a Japanese lung cancer registry study", LUNG CANCER, vol. 50, 2005, pages 227 - 34, XP025286916, DOI: doi:10.1016/j.lungcan.2005.05.021
  • JEMAL A; SIEGEL R; WARD E ET AL.: "Cancer statistics, 2008", CA CANCER J CLIN, vol. 58, 2008, pages 71 - 96
  • NARUKE T; TSUCHIYA R; KONDO H; ASAMURA, H: "Prognosis and survival after resection for bronchogenic carcinoma based on the 1997 TNM-staging classification: the Japanese experience", ANN THORAC SURG, vol. 71, 2001, pages 1759 - 64
  • PFANNSCHMIDT J; MULEY T; BULZEBRUCK H ET AL.: "Prognostic assessment after surgical resection for non-small cell lung cancer: experiences in 2083 patients", LUNG CANCER, vol. 55, 2007, pages 371 - 7, XP005892020, DOI: doi:10.1016/j.lungcan.2006.10.017
  • VAN RENS MT; RIVIERE AB; ELBERS HR; VAN DEN BOSCH JM: "Prognostic assessment of 2,361 patients who underwent pulmonary resection for non-small cell lung cancer, stage I, II, and IIIA", CHEST, vol. 117, 2000, pages 374 - 9
  • HIRSCH FR; WYNES MW; GANDARA DR; BUNN PA JR.: "The tissue is the issue: personalized medicine for non-small cell lung cancer", CLIN CANCER RES, vol. 16, 2010, pages 4909 - 11
  • MINO-KENUDSON M; MARK EJ.: "Reflex testing for epidermal growth factor receptor mutation and anaplastic lymphoma kinase fluorescence in situ hybridization in non-small cell lung cancer", ARCH PATHOL LAB MED, vol. 135, 2011, pages 655 - 64
  • PAO W; GIRARD N.: "New driver mutations in non-small-cell lung cancer", LANCET ONCOL, vol. 12, 2011, pages 175 - 80
  • BRIA E; MILELLA M; CUPPONE F ET AL.: "Outcome of advanced NSCLC patients harboring sensitizing EGFR mutations randomized to EGFR tyrosine kinase inhibitors or chemotherapy as first-line treatment: a meta-analysis", ANN ONCOL, vol. 22, 2011, pages 2277 - 85
  • GERBER DE; MINNA JD: "ALK inhibition for non-small cell lung cancer: from discovery to therapy in record time", CANCER CELL, vol. 18, 2010, pages 548 - 51, XP055043028, DOI: doi:10.1016/j.ccr.2010.11.033
  • SASAKI T; JANNE PA.: "New strategies for treatment of ALK-rearranged non-small cell lung cancers", CLIN CANCER RES, vol. 17, 2011, pages 7213 - 8, XP055090585, DOI: doi:10.1158/1078-0432.CCR-11-1404
  • SHAW AT; SOLOMON B.: "Targeting anaplastic lymphoma kinase in lung cancer", CLIN CANCER RES, vol. 17, 2011, pages 2081 - 6
  • SHAW AT; YEAP BY; SOLOMON BJ ET AL.: "Effect of crizotinib on overall survival in patients with advanced non-small-cell lung cancer harbouring ALK gene rearrangement: a retrospective analysis", LANCET ONCOL, vol. 12, 2011, pages 1004 - 12
  • YOSHIDA A; TSUTA K; NAKAMURA H ET AL.: "Comprehensive histologic analysis of ALK-rearranged lung carcinomas", AM J SURG PATHOL, vol. 35, 2011, pages 1226 - 34
  • HOFMAN V; BONNETAUD C; ILIE MI ET AL.: "Preoperative circulating tumor cell detection using the isolation by size of epithelial tumor cell method for patients with lung cancer is a new prognostic biomarker", CLIN CANCER RES, vol. 17, 2011, pages 827 - 35, XP055055967, DOI: doi:10.1158/1078-0432.CCR-10-0445
  • KREBS MG; SLOANE R; PRIEST L ET AL.: "Evaluation and prognostic significance of circulating tumor cells in patients with non-small-cell lung cancer", J CLIN ONCOL, vol. 29, 2011, pages 1556 - 63, XP055040517, DOI: doi:10.1200/JCO.2010.28.7045
  • KREBS MG; HOU JM; SLOANE R ET AL.: "Analysis of circulating tumor cells in patients with non-small cell lung cancer using epithelial marker-dependent and -independent approaches", J THORAC ONCOL, 14 December 2011 (2011-12-14)
  • PATERLINI-BRECHOT P; BENALI NL.: "Circulating tumor cells (CTC) detection: clinical impact and future directions", CANCER LETT, vol. 253, 2007, pages 180 - 204, XP022144282, DOI: doi:10.1016/j.canlet.2006.12.014
  • YU M; STOTT S; TONER M ET AL.: "Circulating tumor cells: approaches to isolation and characterization", J CELL BIOL, vol. 192, 2011, pages 373 - 82, XP055020222, DOI: doi:10.1083/jcb.201010021
  • MAHESWARAN S; SEQUIST LV; NAGRATH S ET AL.: "Detection of mutations in EGFR in circulating lung-cancer cells", N ENGL J MED, vol. 359, 2008, pages 366 - 77, XP002628814, DOI: doi:10.1056/NEJMOA0800668
  • HOFMAN V; ILIE MI; LONG E ET AL.: "Detection of circulating tumor cells as a prognostic factor in patients undergoing radical surgery for non-small-cell lung carcinoma: comparison of the efficacy of the CellSearch AssayTM and the isolation by size of epithelial tumor cell method", INT J CANCER, vol. 129, 2011, pages 1651 - 60, XP055064899, DOI: doi:10.1002/ijc.25819
  • HOFMAN V; LONG E; ILIE M ET AL.: "Morphological analysis of circulating tumor cells in patients undergoing surgery for non-small cell lung carcinoma using the isolation by size of epithelial tumor cell (ISET) method", CYTOPATHOLOGY, vol. 23, 2012, pages 30 - 8
  • VONA G; SABILE A; LOUHA M ET AL.: "Isolation by size of epithelial tumor cells: a new method for the immunomorphological and molecular characterization of circulating tumor cells", AM J PATHOL, vol. 156, 2000, pages 57 - 63
  • HOU JM; KREBS M; WARD T ET AL., AM J PATHOL, vol. 178, 2011, pages 989 - 96, Retrieved from the Internet <URL:http://www.ncbi.nlm.nih.gov/pubmed/21356352>
  • LECHARPENTIER A; VIELH P; PEREZ-MORENO P ET AL.: "Detection of circulating tumor cells with a hybrid (epithelial/mesenchymal) phenotype in patients with metastatic non-small cell lung cancer", BR J CANCER, vol. 105, 2011, pages 1338 - 41, XP055020091, DOI: doi:10.1038/bjc.2011.405
  • LABABEDE O; MEZIANE M; RICE T.: "Seventh edition of the cancer staging manual and stage grouping of lung cancer: quick reference chart and diagrams", CHEST, vol. 139, 2011, pages 183 - 9
  • TRAVIS WD; BRAMBILLA E; NOGUCHI M ET AL.: "International association for the study of lung cancer/american thoracic society/european respiratory society international multidisciplinary classification of lung adenocarcinoma", J THORAC ONCOL, vol. 6, 2011, pages 244 - 85
  • RODIG SJ; MINO-KENUDSON M; DACIC S ET AL.: "Unique clinicopathologic features characterize ALK-rearranged lung carcinoma in the western population", CLIN CANCER RES, vol. 15, 2009, pages 5216 - 23
  • OGINO S; KAWASAKI T; BRAHMANDAM M ET AL.: "Sensitive sequencing method for KRAS mutation detection by pyrosequencing", J MOL DIAGN, vol. 7, 2005, pages 413 - 21
  • RICHMAN SD; SEYMOUR MT; CHAMBERS P ET AL.: "KRAS and BRAF mutations in advanced colorectal cancer are associated with poor prognosis but do not preclude benefit from oxaliplatin or Irinotecan: Results From the MRC FOCUS Trial", J CLIN ONCOL, vol. 27, 2009, pages 5931 - 7
  • HOFMAN P; ILIE M; HOFMAN V ET AL.: "Immunohistochemistry to identify EGFR mutations or ALK rearrangements in patients with lung adenocarcinoma", ANN ONCOL, 18 November 2011 (2011-11-18)
  • KOIVUNEN JP; MERMEL C; ZEJNULLAHU K ET AL.: "EML4-ALK fusion gene and efficacy of an ALK kinase inhibitor in lung cancer", CLIN CANCER RES, vol. 14, 2008, pages 4275 - 83, XP055090687, DOI: doi:10.1158/1078-0432.CCR-08-0168
  • LEE JK; PARK HS; KIM DW ET AL.: "Comparative analyses of overall survival in patients with anaplastic lymphoma kinase-positive and matched wild-type advanced nonsmall cell lung cancer", CANCER, 15 November 2011 (2011-11-15)
  • WU SG; KUO YW; CHANG YL ET AL.: "EML4-ALK Translocation Predicts Better Outcome in Lung Adenocarcinoma Patients with Wild-Type EGFR", J THORAC ONCOL, vol. 7, 2012, pages 98 - 104
  • YANG P; KULIG K; BOLAND JM ET AL.: "Worse disease-free survival in never-smokers with ALK+ lung adenocarcinoma", J THORAC ONCOL, vol. 7, 2012, pages 90 - 7
  • JUST PA; CAZES A; AUDEBOURG A ET AL.: "Histologic subtypes, immunohistochemistry, FISH or molecular screening for the accurate diagnosis of ALK-rearrangement in lung cancer: A comprehensive study of Caucasian non-smokers", LUNG CANCER, 6 December 2011 (2011-12-06)
  • PAIK JH; CHOI CM; KIM H ET AL.: "Clinicopathologic implication of ALK rearrangement in surgically resected lung cancer A proposal of diagnostic algorithm for ALK-rearranged adenocarcinoma", LUNG CANCER, 28 November 2011 (2011-11-28)
  • SASAKI T; RODIG SJ; CHIRIEAC LR ET AL.: "The biology and treatment of EML4-ALK non-small cell lung cancer", EUR J CANCER, vol. 46, 2010, pages 1773 - 80, XP055399370, DOI: doi:10.1016/j.ejca.2010.04.002
  • POPAT S; GONZALEZ D; MIN T ET AL.: "ALK translocation is associated with ALK immunoreactivity and extensive signet-ring morphology in primary lung adenocarcinoma", LUNG CANCER, 18 August 2011 (2011-08-18)
  • YI ES; BOLAND JM; MALESZEWSKI JJ ET AL.: "Correlation of ICH and FISH for ALK gene rearrangement in non-small cell lung carcinoma: ICH score algorithm for FISH", J THORAC ONCOL, vol. 6, 2011, pages 459 - 65
  • KITAMURA A; HOSODA W; SASAKI E ET AL.: "Immunohistochemical detection of EGFR mutation using mutation-specific antibodies in lung cancer", CLIN CANCER RES, vol. 16, 2010, pages 3349 - 55
  • KREBS MG; HOU JM; WARD TH ET AL.: "Circulating tumor cells: their utility in cancer management and predicting outcomes", THER ADV MED ONCOL, vol. 2, 2010, pages 351 - 65, XP055378183, DOI: doi:10.1177/1758834010378414
  • KATAYAMA R; KHAN TM; BENES C ET AL.: "Therapeutic strategies to overcome crizotinib resistance in non-small cell lung cancers harboring the fusion oncogene EML4-ALK", PROC NATL ACAD SCI U S A, vol. 108, 2011, pages 7535 - 40, XP002672743, DOI: doi:10.1073/pnas.1019559108
  • HEUCKMANN JM; HOLZEL M; SOS ML ET AL.: "ALK mutations conferring differential resistance to structurally diverse ALK inhibitors", CLIN CANCER RES, vol. 17, 2011, pages 7394 - 401
  • KREBS MG; HOU JM; SLOANE R; LANCASHIRE L; PRIEST L; NONAKA D ET AL.: "Analysis of circulating tumor cells in patients with non-small cell lung cancer using epithelial marker-dependent and -independent approaches", J THORAC ONCOL, vol. 7, 2012, pages 306 - 15, XP055367672, DOI: doi:10.1097/JTO.0b013e31823c5c16
  • RHIM AD; MIREK ET; AIELLO NM; MAITRA A; BAILEY JM; MCALLISTER F ET AL.: "EMT and dissemination precede pancreatic tumor formation", CELL, vol. 148, 2012, pages 349 - 61, XP055223014, DOI: doi:10.1016/j.cell.2011.11.025
  • KLEIN C.: "Parallel progression of primary tumours and metastases", NATURE REVIEWS CANCER, vol. 9, 2009, pages 302 - 312
  • ABERLE DR; ADAMS AM; BERG CD; BLACK WC; CLAPP JD; FAGERSTROM RM; GAREEN IF; GATSONIS C; MARCUS PM; SICKS JD: "Reduced lung-cancer mortality with low-dose computed tomographic screening", N ENGL J MED, vol. 365, 2011, pages 395 - 409
  • HOFMAN V; BONNETAUD C; LLIE MI; VIELH P; VIGNAUD JM; FLEJOU JF ET AL.: "Preoperative Circulating Tumor Cell Detection Using the Isolation by Size of Epithelial Tumor Cell Method for Patients with Lung Cancer Is a New Prognostic Biomarker", CLIN CANCER RES, vol. 17, 2011, pages 827 - 35, XP055055967, DOI: doi:10.1158/1078-0432.CCR-10-0445
  • HOFMAN V; LONG E; LLIE M; BONNETAUD C; VIGNAUD JM; FLEJOU JF ET AL.: "Morphological analysis of circulating tumour cells in patients undergoing surgery for non-small cell lung carcinoma using the isolation by size of epithelial tumour cell (ISET) method", CYTOPATHOLOGY, vol. 23, 2012, pages 30 - 8
  • MAZZONE P; MEKHAIL T.: "Current and emerging medical treatments for non-small cell lung cancer: a primer for pulmonologists", RESPIR MED, vol. 106, 2012, pages 473 - 92
  • JEMAL A; SIEGEL R; WARD E; HAO Y; XU J; MURRAY T ET AL., CANCER STATISTICS, 2008
  • CA CANCER J CLIN, vol. 58, 2008, pages 71 - 96
  • METS OM; BUCKENS CF; ZANEN P; ISGUM I; VAN GINNEKEN B; PROKOP M; GIETEMA HA; LAMMERS JW; VLIEGENTHART R; OUDKERK M: "Identification of chronic obstructive pulmonary disease in lung cancer screening computed tomographic scans", JAMA, vol. 306, 2011
  • MUJEZINOVIC F; ALFIREVIC Z: "Procedure-related complications of amniocentesis and chorionic villous sampling: a systematic review", OBSTET GYNECOL, vol. 110, 2007, pages 687 - 694
  • DAN S; WANG W; REN J; LI Y; HU H; XU Z; LAU TK; XIE J; ZHAO W; HUANG H ET AL.: "Clinical application of massively parallel sequencing-based prenatal noninvasive fetal trisomy test for trisomies 21 and 18 in 11105 pregnancies with mixed risk factors", PRENAT DIAGN, vol. 32, 2012, pages 1 - 8
  • ZIMMERMANN B; HILL M; GEMELOS G; DEMKO Z; BANJEVIC M; BANER J; RYAN A; SIGURJONSSON S; CHOPRA N; DODD M ET AL.: "Noninvasive prenatal aneuploidy testing of chromosomes 13, 18, 21, X, and Y, using targeted sequencing of polymorphic loci", PRENAT DIAGN, vol. 32, 2012, pages 1233 - 1241, XP055119823, DOI: doi:10.1002/pd.3993
  • LO YM; LUN FM; CHAN KC; TSUI NB; CHONG KC; LAU TK; LEUNG TY; ZEE BC; CANTOR CR; CHIU RW.: "Digital PCR for the molecular detection of fetal chromosomal aneuploidy", PROC NATL ACAD SCI USA, vol. 104, 2007, pages 13116 - 13121, XP055237304, DOI: doi:10.1073/pnas.0705765104
  • CHIU RW; CHAN KC; GAO Y; LAU VY; ZHENG W; LEUNG TY; FOO CH; XIE B; TSUI NB; LUN FM ET AL.: "Noninvasive prenatal diagnosis of fetal chromosomal aneuploidy by massively parallel genomic sequencing of DNA in maternal plasma", PROC NATL ACAD SCI U S A, vol. 105, 2008, pages 20458 - 20463, XP055284693, DOI: doi:10.1073/pnas.0810641105
  • FAN HC; BLUMENFELD YJ; CHITKARA U; HUDGINS L; QUAKE SR.: "Noninvasive diagnosis of fetal aneuploidy by shotgun sequencing DNA from maternal blood", PROC NATL ACAD SCI U S A, vol. 105, 2008, pages 16266 - 16271, XP002613056, DOI: doi:10.1073/pnas.0808319105
  • VONA G; BEROUD C; BENACHI A; QUENETTE A; BONNEFONT JP; ROMANA S; MUNNICH A; VEKEMANS M; DUMEZ Y; LACOUR B ET AL.: "Enrichment, immunomorphological, and genetic characterization of fetal cells circulating in maternal blood", AM J PATH, vol. 160, 2002, pages 51 - 58, XP002236986
  • MOUAWIA H; SAKER A; JAIS JP; BENACHI A; BUSSIERES L; LACOUR B; BONNEFONT JP; FRYDMAN R; SIMPSON JL; PATERLINI-BRECHOT P.: "Circulating trophoblastic cells provide genetic diagnosis in 63 fetuses at risk for cystic fibrosis or spinal muscular atrophy", REPROD BIOMED ONLINE, vol. 25, 2012, pages 508 - 520, XP028956510, DOI: doi:10.1016/j.rbmo.2012.08.002
  • SHETTLES LB: "Use of the Y chromosome in prenatal sex determination", NATURE, vol. 230, 1971, pages 52 - 53
  • RHINE SA; CAIN JL; CLEARY RE; PALMER CG; THOMPSON JF: "Prenatal sex detection with endocervical smears: successful results utilizing Y-bodyfluorescence", AM J OBSTET GYNECOL, vol. 122, 1975, pages 155 - 160
  • ERGIN T; BALTACI V; ZEYNELOGLU HB; DURAN EH; ERGENELL MH; BATIOGLU S: "Non-invasive early prenatal diagnosis using fluorescent in situ hybridization on transcervical cells: comparison of two different methods for retrieval", EUR J OBSTET GYNECOL REPROD BIOL, vol. 95, 2001, pages 37 - 41, XP002599976
  • CIONI R; BUSSANI C; BUCCIANTINI S; SCARSELLI G: "Fetal cells in a transcervical cell sample collected at 5 weeks of gestation", J MAT-FET NEONAT MED, vol. 18, 2005, pages 271 - 273
  • CIONI R; BUSSANI C; SCARSELLI B; BUCCIANTINI S; MARCHIONNI M; SCARSELLI G: "Comparison of two techniques for transcervical cell sampling performed in the same study population", PRENAT DIAGN, vol. 25, 2005, pages 198 - 202
  • BUSSANI C; SCARSELLI B; CIONI R; BUCCIANTINI S; SCARSELLI G: "Use of the quantitative fluorescent-PCR assay in the study of fetal DNA from micromanipulated transcervical samples", MOL DIAGN, vol. 8, 2004, pages 259 - 263, XP008122852
  • KINGDOM J; SHERLOCK J; RODECK C; ADINOLFI M: "Detection of trophoblast cells in transcervical samples collected by lavage or cytobrush", OBSTET GYNECOL, vol. 86, 1995, pages 283 - 288, XP002986612, DOI: doi:10.1016/0029-7844(95)00127-D
  • MASSARI A; NOVELLI G; COLOSIMO A; SANGIUOLO F, PALKA G; CALABRESE G; CAMURRI L; GHIRARDINI G; MILANI G; GIORLANDINO C ET AL.: "Non-invasive early prenatal molecular diagnosis using retrieved transcervical trophoblast cells", HUM GENET, vol. 97, 1996, pages 150 - 155
  • CHANG SD; LIN SL; CHU KK; HIS BL: "Minimally-invasive early prenatal diagnosis using fluorescence in situ hybridization on samples from uterine lavage", PRENAT DIAGN, vol. 17, 1997, pages 1019 - 1025
  • CHOU MM; LIN SK; HO ES: "Severe limb reduction defects after uterine lavage at 7-8 weeks' gestation", PRENAT DIAGN, vol. 17, 1997, pages 77 - 80
  • ZHANG L; CUI X; SCHMITT K; HUBERT R; NAVIDI W; ARNHEIM N: "Whole genome amplification from a single cell: implications for genetic analysis", PROC NATL ACAD SCI USA, vol. 89, 1992, pages 5847 - 5851, XP002130909, DOI: doi:10.1073/pnas.89.13.5847
  • IMUDIA AN; SUZUKI Y; KILBURN BA; YELIAN FD; DIAMOND MP; ROMERO R; ARMANT DR: "Retrieval of trophoblast cells from the cervical canal for prediction of abnormal pregnancy: a pilot study", HUM REPROD, vol. 24, 2009, pages 2086 - 2092, XP055251574, DOI: doi:10.1093/humrep/dep206
  • COUMANS, F. A. W. ET AL.: "Filter Characteristics Influencing Circulating Tumor Cell Enrichment from Whole Blood", PLOS ONE, vol. 8, no. 4, April 2013 (2013-04-01), pages e61770, XP055293733, DOI: doi:10.1371/journal.pone.0061770
  • COUMANS, F. A. W. ET AL.: "Filtration Parameters Influencing Circulating Tumor Cell Enrichment from Whole Blood", PLOS ONE, vol. 8, no. 4, April 2013 (2013-04-01), pages e61774, XP055329216, DOI: doi:10.1371/journal.pone.0061774

Citation (search report)

  • [XY] WO 9915892 A1 19990401 - FLINDERS TECHNOLOGIES PTY LTD [AU], et al
  • [XY] US 2005049793 A1 20050303 - PATERLINI-BRECHOT PATRIZIA [FR]
  • [Y] WO 2006018849 A2 20060223 - MONALIZA MEDICAL LTD [IL], et al
  • [X] US 2009317797 A1 20091224 - PATERLINI PATRIZIA [FR], et al
  • [X] VONA G ET AL: "Enrichment, immunomorphological, and genetic characterization of fetal cells circulating in maternal blood", AMERICAN JOURNAL OF PATHOLOGY; [10640], ELSEVIER INC, US, vol. 160, no. 1, 1 January 2002 (2002-01-01), pages 51 - 58, XP002236986, ISSN: 0002-9440

Designated contracting state (EPC)

AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

DOCDB simple family (publication)

US 2013316347 A1 20131128; AU 2013265267 A1 20141218; AU 2013265267 B2 20180802; AU 2013265267 C1 20190131; AU 2018247219 A1 20181101; AU 2018247219 B2 20200820; BR 112014029181 A2 20180508; BR 112014029181 B1 20220823; BR 112014029181 B8 20220906; BR 112014029181 B8 20221108; BR 112014029181 B8 20221122; CA 2874048 A1 20131128; CA 2874048 C 20210511; CA 3112123 A1 20131128; CN 104685356 A 20150603; CN 104685356 B 20181019; CN 109517895 A 20190326; CN 109517895 B 20220510; EP 2856153 A1 20150408; EP 2856153 B1 20180117; EP 3351935 A1 20180725; EP 3351935 B1 20200812; EP 3745126 A1 20201202; ES 2666594 T3 20180507; HK 1209186 A1 20160324; IL 235854 A0 20150129; JP 2015524054 A 20150820; JP 2019074538 A 20190516; JP 6761850 B2 20200930; MX 2014014245 A 20150617; RU 2014152244 A 20160720; RU 2017142155 A 20190213; RU 2017142155 A3 20210128; RU 2641595 C2 20180118; RU 2765808 C2 20220203; US 2020264166 A1 20200820; WO 2013174948 A1 20131128

DOCDB simple family (application)

US 201313901063 A 20130523; AU 2013265267 A 20130523; AU 2018247219 A 20181010; BR 112014029181 A 20130523; CA 2874048 A 20130523; CA 3112123 A 20130523; CN 201380039782 A 20130523; CN 201811062672 A 20130523; EP 13724594 A 20130523; EP 17210576 A 20130523; EP 2013060671 W 20130523; EP 20183050 A 20130523; ES 13724594 T 20130523; HK 15109863 A 20151008; IL 23585414 A 20141123; JP 2015513193 A 20130523; JP 2018248522 A 20181228; MX 2014014245 A 20130523; RU 2014152244 A 20130523; RU 2017142155 A 20130523; US 202016746443 A 20200117