EP 3367785 A4 20190814 - COMPOSITIONS AND METHODS FOR CHIMERIC EMBRYO-ASSISTED ORGAN PRODUCTION
Title (en)
COMPOSITIONS AND METHODS FOR CHIMERIC EMBRYO-ASSISTED ORGAN PRODUCTION
Title (de)
ZUSAMMENSETZUNGEN UND VERFAHREN ZUR CHIMÄREN EMBRYOUNTERSTÜTZTEN ORGANPRODUKTION
Title (fr)
COMPOSITIONS ET PROCÉDÉS DE PRODUCTION D'ORGANE ASSISTÉ PAR UN EMBRYON CHIMÈRE
Publication
Application
Priority
- US 201562246926 P 20151027
- US 201562246927 P 20151027
- US 201562246929 P 20151027
- US 201562246947 P 20151027
- US 201562247092 P 20151027
- US 201562247096 P 20151027
- US 201562247115 P 20151027
- US 201562247117 P 20151027
- US 201562247118 P 20151027
- US 201562247122 P 20151027
- US 2016059206 W 20161027
Abstract (en)
[origin: WO2017075276A2] Human or humanized tissues and organs suitable for transplant are disclosed herein. Gene editing of a host animal provides a niche for complementation of the missing genetic information by donor stem cells. Editing of a host genome to knock out or disrupt genes responsible for the growth and/or differentiation of a target organ and injecting that animal at an embryo stage with donor stem cells to complement the missing genetic information for the growth and development of the organ. The result is a chimeric animal in which the complemented tissue (human/humanized organ) matches the genotype and phenotype of the donor. Such organs may be made in a single generation and the stem cell may be taken or generated from the patient's own body. As disclosed herein, it is possible to do so by simultaneously editing multiple genes in a cell or embryo creating a "niche" for the complemented tissue. Multiple genes can be targeted for editing using targeted nucleases and homology directed repair (HDR) templates in vertebrate cells or embryos.
IPC 8 full level
A01K 67/027 (2006.01); A01N 1/02 (2006.01); A61D 19/04 (2006.01); C12N 15/85 (2006.01); C12N 15/90 (2006.01)
CPC (source: EP KR)
A01K 67/027 (2013.01 - KR); A01K 67/0276 (2013.01 - EP KR); C12N 15/907 (2013.01 - EP KR); A01K 2217/075 (2013.01 - EP KR); A01K 2227/108 (2013.01 - EP KR); A01K 2267/025 (2013.01 - EP KR)
Citation (search report)
- [A] US 2010122360 A1 20100513 - NAKAUCHI HIROMITSU [JP], et al
- [XYI] PREETHI SWAMINATHAN: "Human Stem Cell Complementation in PITX3 Null Porcine Blastocysts: Lens Development", December 2014 (2014-12-01), XP055493473, Retrieved from the Internet <URL:https://conservancy.umn.edu/bitstream/handle/11299/185108/Swaminathan_umn_0130M_15713.pdf?sequence=1> [retrieved on 20180718]
- [Y] XIANLONG WANG ET AL: "Efficient CRISPR/Cas9-mediated biallelic gene disruption and site-specific knockin after rapid selection of highly active sgRNAs in pigs", SCIENTIFIC REPORTS, vol. 5, no. 1, 21 August 2015 (2015-08-21), XP055551206, DOI: 10.1038/srep13348
- [Y] MADHUSUDANA GIRIJA SANAL: "Future of liver transplantation: Non-human primates for patient-specific organs from induced pluripotent stem cells", WORLD JOURNAL OF GASTROENTEROLOGY, vol. 17, no. 32, 2011, CN, pages 3684, XP055602899, ISSN: 1007-9327, DOI: 10.3748/wjg.v17.i32.3684
- [Y] YUYU NIU ET AL: "Generation of Gene-Modified Cynomolgus Monkey via Cas9/RNA-Mediated Gene Targeting in One-Cell Embryos", CELL, vol. 156, no. 4, February 2014 (2014-02-01), AMSTERDAM, NL, pages 836 - 843, XP055422516, ISSN: 0092-8674, DOI: 10.1016/j.cell.2014.01.027
- See references of WO 2017075276A2
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
WO 2017075276 A2 20170504; WO 2017075276 A3 20170601; AU 2016344152 A1 20180524; CA 3003652 A1 20170504; CN 108697067 A 20181023; EP 3367785 A2 20180905; EP 3367785 A4 20190814; JP 2019502400 A 20190131; KR 20180100303 A 20180910
DOCDB simple family (application)
US 2016059206 W 20161027; AU 2016344152 A 20161027; CA 3003652 A 20161027; CN 201680074878 A 20161027; EP 16860830 A 20161027; JP 2018542691 A 20161027; KR 20187014498 A 20161027