EP 3371194 B1 20201230 - PROTEIN SEQUENCING METHOD
Title (en)
PROTEIN SEQUENCING METHOD
Title (de)
PROTEINSEQUENZIERUNGSVERFAHREN
Title (fr)
PROCÉDÉ DE SÉQUENÇAGE DE PROTÉINES
Publication
Application
Priority
- US 201562242619 P 20151016
- CA 2016051204 W 20161017
Abstract (en)
[origin: WO2017063093A1] Described are optical methods and reagents for sequencing polypeptides. A probe that exhibits different spectral properties when conjugated to different N-terminal amino acids is conjugated to the N-terminal amino acid of a polypeptide. Sequentially detecting one or more spectral properties of the probe conjugated to the N-terminal amino acid and cleaving the N-terminal amino acid produces sequence information of the polypeptide. The use of super-resolution microscopy allows for the massively parallel sequencing of individual polypeptide molecules in situ such as within a cell. Also described are probes comprising hydroxymethyl rhodamine green, an isothiocyanate group and a protecting group.
IPC 8 full level
G01N 33/68 (2006.01); C07D 493/10 (2006.01); C40B 20/04 (2006.01)
CPC (source: EP US)
C07D 493/10 (2013.01 - EP US); C40B 20/04 (2013.01 - EP US); G01N 33/6824 (2013.01 - EP US); Y02P 20/55 (2015.11 - EP US)
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
WO 2017063093 A1 20170420; CA 3040566 A1 20170420; EP 3371194 A1 20180912; EP 3371194 A4 20190612; EP 3371194 B1 20201230; US 11268963 B2 20220308; US 2018299460 A1 20181018; US 2022155316 A1 20220519
DOCDB simple family (application)
CA 2016051204 W 20161017; CA 3040566 A 20161017; EP 16854691 A 20161017; US 201615768602 A 20161017; US 202217588754 A 20220131