EP 3423463 A4 20191225 - SYSTEM AND PROCESS FOR DATA-DRIVEN DESIGN, SYNTHESIS, AND APPLICATION OF MOLECULAR PROBES
Title (en)
SYSTEM AND PROCESS FOR DATA-DRIVEN DESIGN, SYNTHESIS, AND APPLICATION OF MOLECULAR PROBES
Title (de)
SYSTEM UND VERFAHREN ZUM DATENGESTEUERTEN ENTWURF, ZUR SYNTHESE UND ANWENDUNG VON MOLEKULAREN SONDEN
Title (fr)
SYSTÈME ET PROCÉDÉ POUR UNE CONCEPTION ET UNE SYNTHÈSE GUIDÉES PAR LES DONNÉES ET APPLICATION DE SONDES MOLÉCULAIRES
Publication
Application
Priority
- US 201662302078 P 20160301
- CA 2017050273 W 20170301
Abstract (en)
[origin: WO2017147702A1] The disclosure provides methods and systems for designing and synthesizing probes to capture a representative sample of genomic variants of a target genome from a sample. The methods include providing a multiple sequence alignment (MSA), designing a plurality of representative subsequences, and optionally synthesizing a nucleic acid probe. The designing step can comprise designating a plurality of intervals in the MSA, shifting start positions for each MSA subset, clustering the aligned subsequences within each adjusted subset, and determining a representative sequence for each reduced MSA subset. The disclosure also encompasses methods of isolating a plurality of nucleic acid variants of a targeted genomic subregion from a sample using the disclosed probe design, as well as the probe compositions themselves.
IPC 8 full level
C07H 21/00 (2006.01); C07H 21/04 (2006.01); C12N 15/10 (2006.01); C12Q 1/68 (2018.01); C40B 30/04 (2006.01); G16B 25/20 (2019.01); G16B 30/10 (2019.01)
CPC (source: EP US)
C07H 21/04 (2013.01 - EP US); C12N 15/10 (2013.01 - EP US); C12N 15/1003 (2013.01 - EP US); C12N 15/1089 (2013.01 - EP US); C12Q 1/6806 (2013.01 - US); C40B 30/04 (2013.01 - US); G16B 25/00 (2019.02 - EP US); G16B 25/20 (2019.02 - EP US); G16B 30/00 (2019.02 - EP US); G16B 30/10 (2019.02 - EP US); G16B 50/00 (2019.02 - US)
C-Set (source: US)
Citation (search report)
- [XY] HUEMAN JAIMES-DÍAZ ET AL: "In Silico Genomic Fingerprints of the Bacillus anthracis Group Obtained by Virtual Hybridization", MICROARRAYS, vol. 4, no. 1, 1 March 2015 (2015-03-01), pages 84 - 97, XP055637813, ISSN: 2076-3905, DOI: 10.3390/microarrays4010084
- [XY] HUEMAN JAIMES-DÃAZ ET AL: "Bacterial classification using genomic fingerprints obtained by virtual hybridization", JOURNAL OF MICROBIOLOGICAL METHODS, ELSEVIER, AMSTERDAM, NL, vol. 87, no. 3, 24 August 2011 (2011-08-24), pages 286 - 294, XP028109157, ISSN: 0167-7012, [retrieved on 20110901], DOI: 10.1016/J.MIMET.2011.08.014
- [XY] A IVSHINA ET AL: "Mapping of genomic segments of influenza B virus strains by an oligonucleotide microarray method.", J CLIN MICROBIOL., 31 December 2004 (2004-12-31), pages 5793 - 5801, XP055637820
- See also references of WO 2017147702A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
WO 2017147702 A1 20170908; CA 3016222 A1 20170908; CN 109071590 A 20181221; CN 109071590 B 20230808; EP 3423463 A1 20190109; EP 3423463 A4 20191225; US 11728009 B2 20230815; US 2019080049 A1 20190314
DOCDB simple family (application)
CA 2017050273 W 20170301; CA 3016222 A 20170301; CN 201780024591 A 20170301; EP 17759041 A 20170301; US 201716081879 A 20170301