EP 3472204 A1 20190424 - METHOD FOR PREPARING ANTI-AMH ANTIBODIES AND USES OF SAME
Title (en)
METHOD FOR PREPARING ANTI-AMH ANTIBODIES AND USES OF SAME
Title (de)
VERFAHREN ZUR HERSTELLUNG VON ANTI-AMH-ANTIKÖRPERN UND VERWENDUNGEN DAVON
Title (fr)
PROCÉDÉ DE PRÉPARATION D'ANTICORPS ANTI-AMH ET LEURS UTILISATIONS
Publication
Application
Priority
- EP 16175041 A 20160617
- EP 2017064759 W 20170616
Abstract (en)
[origin: WO2017216334A1] The present invention relates to a method for preparing mammalian anti-AMH antibodies comprising the steps of: (i) immunising an animal with a polypeptide of AMH or a polynucleotide coding for this polypeptide of AMH, said polypeptide of AMH comprising at least the 99 amino acids of sequence SEQ ID N°1 or of a sequence having at least 75% identity with sequence SEQ ID N°1, and at most the 560 amino acids of sequence SEQ ID N°2 or of a sequence having at least 75% identity with sequence SEQ ID N°2, (ii) preparing hybridomas from cells of a lymphoid organ of the animal having received the immunogen, (iii) selecting hybridomas secreting antibodies recognising a polypeptide of AMH comprising at least the 99 amino acids of sequence SEQ ID N°1 or of a sequence having at least 75% identity with sequence SEQ ID N°1, and at most the 255 amino acids of sequence SEQ ID N°8 or of a sequence having at least 75% identity with sequence SEQ ID N°8, but recognising neither (a) a polypeptide of AMH comprising at least the 131 amino acids of sequence SEQ ID N°13 or of a sequence having at least 75% identity with sequence SEQ ID N°13 and at most the 20,156 amino acids of sequence SEQ ID N°11 or of a sequence having at least 75% identity with sequence SEQ ID N°11, nor (b) any linear epitope located in sequence SEQ ID N°1 or a sequence having at least 75% identity with sequence SEQ ID N°1, and (iv) producing the antibodies. The invention also relates to antibodies, antibody fragments and the use of same for assaying AMH, particularly in fertility.
IPC 8 full level
C07K 16/26 (2006.01); G01N 33/53 (2006.01)
CPC (source: EP RU US)
C07K 16/26 (2013.01 - EP RU US); G01N 33/53 (2013.01 - RU); G01N 33/689 (2013.01 - EP RU US); G01N 33/74 (2013.01 - EP RU US); C07K 2317/10 (2013.01 - US); C07K 2317/34 (2013.01 - EP US); G01N 2333/575 (2013.01 - US); G01N 2800/367 (2013.01 - EP US)
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
EP 3257867 A1 20171220; AU 2017285276 A1 20190131; AU 2017285276 B2 20240718; BR 112018076089 A2 20190326; CN 109563163 A 20190402; EP 3472204 A1 20190424; JP 2019528676 A 20191017; JP 7057759 B2 20220420; RU 2019101062 A 20200717; RU 2019101062 A3 20201014; RU 2764198 C2 20220114; US 11225518 B2 20220118; US 2019211096 A1 20190711; WO 2017216334 A1 20171221
DOCDB simple family (application)
EP 16175041 A 20160617; AU 2017285276 A 20170616; BR 112018076089 A 20170616; CN 201780049802 A 20170616; EP 17731864 A 20170616; EP 2017064759 W 20170616; JP 2018565664 A 20170616; RU 2019101062 A 20170616; US 201716310802 A 20170616