EP 3480216 A1 20190508 - ANTI-PCSK9 MONOCLONAL ANTIBODY
Title (en)
ANTI-PCSK9 MONOCLONAL ANTIBODY
Title (de)
MONOKLONALER ANTI-PCSK9-ANTIKÖRPER
Title (fr)
ANTICORPS MONOCLONAL ANTI-PCSK9
Publication
Application
Priority
- CN 201710052879 A 20170122
- CN 2017119822 W 20171229
Abstract (en)
The application discloses an anti-Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) monoclonal antibody, an amino acid sequence comprising coded antibody variable regions and Complementarity-Determining Regions (CDR), the obtaining method , and application of monoclonal antibody. The anti-PCSK9 monoclonal antibodies are screened from a phage antibody library; affinity maturation is achieved by using a method that the phage antibody library is established by means of chain displacement; After screening the light chain CDR1, 2, 3 Mutant Library of monoclonal antibodies obtained from primary screening; monoclonal antibodies having high affinity are selected; then screening the mutant libraries of heavy chain CDR1, 2 , 3 regions ; and finally the monoclonal antibodies having high affinity are screened. The PCSK9 antibody obtained by the application has good affinity to PCSK9 and can inhibit the binding of PCSK9 with its ligand and can be used for treating dyslipidemia, cardiovascular and cerebrovascular diseases and thrombotic occlusion diseases.
IPC 8 full level
C07K 16/40 (2006.01); A61K 39/395 (2006.01); A61P 3/06 (2006.01); A61P 7/02 (2006.01); A61P 9/00 (2006.01); A61P 9/10 (2006.01); C12N 15/13 (2006.01); G01N 33/573 (2006.01); G01N 33/577 (2006.01)
CPC (source: CN EA EP KR US)
A61K 35/17 (2013.01 - CN EA EP KR US); A61P 9/00 (2018.01 - EP); C07K 16/40 (2013.01 - CN EA EP KR US); A61K 39/00 (2013.01 - CN EA EP KR); A61K 2039/505 (2013.01 - CN EA EP KR); C07K 2317/14 (2013.01 - US); C07K 2317/24 (2013.01 - CN EA EP KR); C07K 2317/565 (2013.01 - CN EA EP KR US); C07K 2317/92 (2013.01 - CN EA EP KR)
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
EP 3480216 A1 20190508; EP 3480216 A4 20200701; AU 2017394444 A1 20190221; AU 2017394444 B2 20200409; CA 3032506 A1 20180726; CA 3032506 C 20230822; CN 106749670 A 20170531; CN 106749670 B 20180612; CN 107698679 A 20180216; CN 107698679 B 20190301; CN 107698680 A 20180216; CN 107698680 B 20190301; EA 039191 B1 20211215; EA 201990170 A1 20190731; JP 2019531057 A 20191031; JP 6734467 B2 20200805; KR 102257462 B1 20210531; KR 20190025980 A 20190312; US 11390688 B2 20220719; US 2021277146 A1 20210909; WO 2018133649 A1 20180726
DOCDB simple family (application)
EP 17893120 A 20171229; AU 2017394444 A 20171229; CA 3032506 A 20171229; CN 201710052879 A 20170122; CN 201711161960 A 20170122; CN 201711161968 A 20170122; CN 2017119822 W 20171229; EA 201990170 A 20171229; JP 2019505510 A 20171229; KR 20197003329 A 20171229; US 201716321472 A 20171229