EP 3487983 A1 20190529 - SEPARATION OF ENZYMES FROM TRICHODERMA REESEI BY FILTER PRESS AND TANGENTIAL FILTRATION ON A CERAMIC MEMBRANE
Title (en)
SEPARATION OF ENZYMES FROM TRICHODERMA REESEI BY FILTER PRESS AND TANGENTIAL FILTRATION ON A CERAMIC MEMBRANE
Title (de)
TRENNUNG VON ENZYMEN AUS TRICHODERMA REESEI DURCH EINE FILTERPRESSE UND TANGENTIALFILTRATION AUF EINER KERAMISCHEN MEMBRAN
Title (fr)
SEPARATION DES ENZYMES ISSUES DE TRICHODERMA REESEI PAR FILTRE PRESSE ET FILTRATION TANGENTIELLE SUR MEMBRANE CERAMIQUE
Publication
Application
Priority
- FR 1657030 A 20160722
- EP 2017067470 W 20170711
Abstract (en)
[origin: WO2018015228A1] The invention relates to a method for separating, from a culture medium, an enzymatic cocktail and the fungus Trichoderma Reesei, the culture medium resulting from enzyme production by the fungus, method in which: said culture medium is subjected, in a period of no longer than 30 h from the halting of production, to a separation on a filter press lined with a fabric having a porosity of 3-20 μm, so as to obtain a filtrate having a corrected optical density OD at 600 nm of less than 2.5; and the liquid phase obtained is subjected to tangential micro-filtration on a ceramic membrane having a cut-off limit of 0.5 to 1.4 μm, so that the corrected OD at 600 nm does not exceed 0.1.
IPC 8 full level
C12N 1/02 (2006.01); C07K 1/34 (2006.01); C12N 1/14 (2006.01); C12N 9/42 (2006.01); C12P 21/00 (2006.01)
CPC (source: EP US)
C07K 1/34 (2013.01 - US); C12N 1/02 (2013.01 - EP US); C12N 1/14 (2013.01 - EP US); C12N 9/2437 (2013.01 - EP US); C12P 7/06 (2013.01 - US); C12P 21/00 (2013.01 - EP); C12P 21/00 (2013.01 - US); Y02E 50/10 (2013.01 - EP)
Citation (search report)
See references of WO 2018015228A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
WO 2018015228 A1 20180125; AU 2017299082 A1 20190314; AU 2017299082 A8 20190516; AU 2017299082 B2 20230330; BR 112019000802 A2 20190424; CA 3031396 A1 20180125; CN 109563468 A 20190402; CN 109563468 B 20230331; EP 3487983 A1 20190529; FR 3054141 A1 20180126; FR 3054141 B1 20180713; JP 2019520842 A 20190725; JP 6942406 B2 20210929; MX 2019000502 A 20190801; US 11274122 B2 20220315; US 2020031864 A1 20200130; ZA 201900023 B 20190925
DOCDB simple family (application)
EP 2017067470 W 20170711; AU 2017299082 A 20170711; BR 112019000802 A 20170711; CA 3031396 A 20170711; CN 201780045492 A 20170711; EP 17737287 A 20170711; FR 1657030 A 20160722; JP 2019502202 A 20170711; MX 2019000502 A 20170711; US 201716318446 A 20170711; ZA 201900023 A 20190103