EP 3500684 A1 20190626 - OPTICALLY-BASED NANOPORE SEQUENCING USING QUENCHING AGENTS
Title (en)
OPTICALLY-BASED NANOPORE SEQUENCING USING QUENCHING AGENTS
Title (de)
OPTISCHE NANOPORENSEQUENZIERUNG MIT QUENCHERN
Title (fr)
SÉQUENÇAGE PAR NANOPORES À BASE OPTIQUE À L'AIDE D'AGENTS D'EXTINCTION
Publication
Application
Priority
- US 201662377409 P 20160819
- US 2017043772 W 20170725
Abstract (en)
[origin: WO2018034807A1] The present invention is directed to methods and compositions for optically based polynucleotide analysis using nanopores wherein quenching agents are used to reduce or eliminate spurious optical signals generated by polynucleotide labels outside of detection zones. In some embodiments, the invention may be implemented with the following steps: translocating a polynucleotide through a nanopore, wherein different kinds of nucleotides of the polynucleotide are labeled with different flurorescent labels that generate distinguishable fluorescent signals and wherein the nanopore constrains the nucleotides to move single file through a detection zone; exciting the fluorescent labels; quenching fluorescent signals from excited fluorescent labels outside of the detection zone using one or more non-fluorescent quenching agents; detecting fluorescent signals emitted from the fluorescent labels as the fluorescent labels pass through the detection zone; and determining a sequence of nucleotides from the detected fluorescent signals.
IPC 8 full level
C12Q 1/68 (2018.01)
CPC (source: EP)
C12Q 1/68 (2013.01); C12Q 1/6869 (2013.01)
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
WO 2018034807 A1 20180222; AU 2017312746 A1 20190131; CA 3031812 A1 20180222; CN 109804087 A 20190524; EP 3500684 A1 20190626; EP 3500684 A4 20200226; JP 2019528065 A 20191010
DOCDB simple family (application)
US 2017043772 W 20170725; AU 2017312746 A 20170725; CA 3031812 A 20170725; CN 201780062870 A 20170725; EP 17841831 A 20170725; JP 2019509522 A 20170725