EP 3507301 A4 20201028 - SEROLOGIC ASSAY OF LIVER FIBROSIS
Title (en)
SEROLOGIC ASSAY OF LIVER FIBROSIS
Title (de)
SEROLOGISCHER TEST FÜR LEBERFIBROSE
Title (fr)
DOSAGE SÉROLOGIQUE DE FIBROSE HÉPATIQUE
Publication
Application
Priority
- US 201662383020 P 20160902
- US 2017049563 W 20170831
Abstract (en)
[origin: WO2018045145A1] Provided are in vitro serologic methods of assessing the presence of, and assessing the progression of, liver fibrosis in a subject. Also provided are methods of assessing efficacy of an agent for the treatment of liver fibrosis, and methods of treating liver fibrosis. The methods involve quantitatively measuring di-sialylated and mono-sialylated O-glycoforms of a peptide fragment of hemopexin (HPX) in a test serum sample obtained from a test subject, and comparing the measured amounts of di-sialylated and mono-sialylated O-glycoforms of the peptide fragment of hemopexin to a reference amount. In certain embodiments, the measuring is performed using LC-MS/MS-MRM (liquid chromatography/tandem mass spectrometry/multiple reaction monitoring). In certain embodiments, the measuring is performed using LC/MS3 (liquid chromatography with triple-stage mass spectrometric detection).
IPC 8 full level
C07K 14/805 (2006.01); G01N 33/68 (2006.01)
CPC (source: EP US)
C07K 14/47 (2013.01 - EP US); G01N 33/6827 (2013.01 - US); G01N 33/6848 (2013.01 - US); G01N 33/6893 (2013.01 - EP US); G01N 2800/085 (2013.01 - EP US); G01N 2800/52 (2013.01 - EP US)
Citation (search report)
- [XP] MILOSLAV SANDA ET AL: "Increased sialylation of site specific O-glycoforms of hemopexin in liver disease", CLINICAL PROTEOMICS, SPRINGER, US, vol. 13, no. 1, 21 September 2016 (2016-09-21), pages 1 - 12, XP021264936, ISSN: 1542-6416, DOI: 10.1186/S12014-016-9125-X
- [A] JULIUS BENICKY ET AL: "Quantification of Fucosylated Hemopexin and Complement Factor H in Plasma of Patients with Liver Disease", ANALYTICAL CHEMISTRY, vol. 86, no. 21, 4 November 2014 (2014-11-04), pages 10716 - 10723, XP055728779, ISSN: 0003-2700, DOI: 10.1021/ac502727s
- See references of WO 2018045145A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
WO 2018045145 A1 20180308; EP 3507301 A1 20190710; EP 3507301 A4 20201028; US 11035865 B2 20210615; US 2019242907 A1 20190808
DOCDB simple family (application)
US 2017049563 W 20170831; EP 17847535 A 20170831; US 201716329926 A 20170831