EP 3578667 A1 20191211 - METHODS FOR CHARACTERIZING DNA SEQUENCE COMPOSITION IN A GENOME

Title (en)

METHODS FOR CHARACTERIZING DNA SEQUENCE COMPOSITION IN A GENOME

Title (de)

VERFAHREN ZUR CHARAKTERISIERUNG DER DNA-SEQUENZ-ZUSAMMENSETZUNG IN EINEM GENOM

Title (fr)

PROCÉDÉS DE CARACTÉRISATION DE COMPOSITION DE SÉQUENCES D'ADN DANS UN GÉNOME

Publication

EP 3578667 A1 20191211 (EN)

Application

EP 19179142 A 20140417

Priority

US 201361812876 P 20130417
US 201361813001 P 20130417
EP 14724967 A 20140417
US 2014034476 W 20140417

Abstract (en)

Methods for the high-throughput analysis of transgenic events are herein disclosed. The methods use libraries of sheared genomic DNA ligated to specialized adapters and pooled for sequence analysis and comparison to known genomic and insert sequence. The method finds use in detecting characterizing insertion site, transgene integrity, and transgene copy number.

IPC 8 full level

C12Q 1/68 (2018.01); C12Q 1/6806 (2018.01); C12Q 1/6869 (2018.01); C12Q 1/6895 (2018.01)

CPC (source: EP US)

C12Q 1/6806 (2013.01 - EP US); C12Q 1/6869 (2013.01 - EP US); C12Q 1/6895 (2013.01 - EP US); C12Q 2600/13 (2013.01 - EP US)

Citation (applicant)

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Citation (search report)

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[I] DORIAN FABIAN URBANSKI ET AL: "Genome-wide LORE1 retrotransposon mutagenesis and high-throughput insertion detection in Lotus japonicus", THE PLANT JOURNAL, vol. 69, no. 4, 1 December 2011 (2011-12-01), pages 731 - 741, XP055128452, ISSN: 0960-7412, DOI: 10.1111/j.1365-313X.2011.04827.x
[Y] JENNIFER MONSON-MILLER ET AL: "Reference genome-independent assessment of mutation density using restriction enzyme-phased sequencing", BMC GENOMICS, BIOMED CENTRAL LTD, LONDON, UK, vol. 13, no. 1, 14 February 2012 (2012-02-14), pages 72, XP021117861, ISSN: 1471-2164, DOI: 10.1186/1471-2164-13-72
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Designated contracting state (EPC)

AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

DOCDB simple family (publication)

US 2014315726 A1 20141023; US 9797001 B2 20171024; BR 112015026499 A2 20170725; BR 112015026499 B1 20220621; CA 2908361 A1 20141023; CA 2908361 C 20230926; CN 105492625 A 20160413; CN 105492625 B 20200407; CN 111383715 A 20200707; EP 2986738 A1 20160224; EP 2986738 B1 20190724; EP 3578667 A1 20191211; EP 3578667 B1 20231004; MX 2015014583 A 20160629; MX 2019004588 A 20190812; MX 364309 B 20190422; US 10487352 B2 20191126; US 10941436 B2 20210309; US 11702685 B2 20230718; US 2018016626 A1 20180118; US 2020032320 A1 20200130; US 2021238651 A1 20210805; WO 2014172529 A1 20141023

DOCDB simple family (application)

US 201414255144 A 20140417; BR 112015026499 A 20140417; CA 2908361 A 20140417; CN 201480021755 A 20140417; CN 202010157360 A 20140417; EP 14724967 A 20140417; EP 19179142 A 20140417; MX 2015014583 A 20140417; MX 2019004588 A 20151015; US 2014034476 W 20140417; US 201715711403 A 20170921; US 201916598341 A 20191010; US 202117169609 A 20210208