EP 3588089 B1 20210310 - CELL-BASED METHODS FOR COUPLING PROTEIN INTERACTIONS AND BINDING MOLECULE SELECTION AND DIVERSIFICATION
Title (en)
CELL-BASED METHODS FOR COUPLING PROTEIN INTERACTIONS AND BINDING MOLECULE SELECTION AND DIVERSIFICATION
Title (de)
ZELLBASIERTE VERFAHREN ZUR KOPPLUNG VON PROTEININTERAKTIONEN UND BINDUNGSMOLEKÜLAUSWAHLEN UND -DIVERSIFIZIERUNGEN
Title (fr)
MÉTHODES DE TYPE CELLULAIRE POUR LE COUPLAGE D'INTERACTIONS PROTÉIQUES ET SÉLECTION ET DIVERSIFICATION DE MOLÉCULES DE LIAISON
Publication
Application
Priority
- US 201361771562 P 20130301
- EP 14722391 A 20140303
- PT 2014000016 W 20140303
Abstract (en)
[origin: US2014249044A1] The invention relates to cell-based methods for diversifying, expressing and selecting binding molecules, e.g., antibodies, and target molecules to which they bind, all of which are expressed in the same cell. The target molecule can be a member of a ligand binding pair comprising a cell-surface expressed ligand binding receptor molecule and its cognate ligand, which interact within the cell. The methods provide retaining either the antibody or its target in a cell organelle as the site of binding and interaction. By performance of the methods, the binding or non-binding of the antibody to its target molecule within the cell produces a cell phenotype that is detectable at the cell surface via high throughput assays, e.g., flow cytometry. The methods are particularly useful for generating, recovering and providing antibodies that have optimal target molecule binding properties or activities for potential therapeutic use. Methods for generating diversity in such antibodies are also provided.
IPC 8 full level
G01N 33/537 (2006.01); G01N 33/566 (2006.01)
CPC (source: EP US)
C12N 15/1024 (2013.01 - EP US); C12N 15/1037 (2013.01 - EP US); C12N 15/1055 (2013.01 - EP US); G01N 33/537 (2013.01 - EP US); G01N 33/566 (2013.01 - EP US); G01N 2500/02 (2013.01 - EP US); G01N 2500/10 (2013.01 - EP US)
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
US 2014249044 A1 20140904; US 9487773 B2 20161108; EP 2962103 A2 20160106; EP 3588089 A1 20200101; EP 3588089 B1 20210310; ES 2872048 T3 20211102; JP 2016511405 A 20160414; PT 3588089 T 20210602; WO 2014133405 A2 20140904; WO 2014133405 A3 20150226
DOCDB simple family (application)
US 201414193679 A 20140228; EP 14722391 A 20140303; EP 19020049 A 20140303; ES 19020049 T 20140303; JP 2015560136 A 20140303; PT 19020049 T 20140303; PT 2014000016 W 20140303