EP 3596224 A1 20200122 - METHOD OF DETERMINATION OF HYALURONIC ACID
Title (en)
METHOD OF DETERMINATION OF HYALURONIC ACID
Title (de)
VERFAHREN ZUR BESTIMMUNG VON HYALURONSÄURE
Title (fr)
PROCÉDÉ DE DÉTERMINATION DE LA PRÉSENCE D'ACIDE HYALURONIQUE
Publication
Application
Priority
- CZ 2017145 A 20170316
- CZ 2018050009 W 20180315
Abstract (en)
[origin: WO2018166545A1] The invention relates to a method of determination of hyaluronic acid in complex samples by cleavage with SpnHyl enzyme followed by the reaction with 3-methyl-2-benzothiazolinone hydrazone (MBTH) to form a coloured product analysed by spectrophotometry. In case of a high content of glycosaminoglycans, these can be separated by precipitation before the cleavage, e.g. with the use of cetyltrimethylammomumbromide (CTAB) in acetate buffer and salt medium. In case of a high content of reducing sugars, e.g. glucose, their influence can be eliminated, after removing the glycosaminoglycans and before the cleavage, by precipitating hyaluronic acid from the formed supernatant and then redissolving this sediment in acetate buffer and performing the cleavage step.
IPC 8 full level
C12P 19/04 (2006.01); C08B 37/08 (2006.01); C12P 19/12 (2006.01); C12Q 1/527 (2006.01)
CPC (source: EP)
C08B 37/0072 (2013.01); C12Q 1/527 (2013.01); C12Y 402/02001 (2013.01)
Citation (search report)
See references of WO 2018166545A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
DOCDB simple family (application)
CZ 2018050009 W 20180315; EP 18722381 A 20180315