EP 3600425 A4 20201223 - METHODS FOR EVALUATING TUMOR CELL SPHEROIDS USING 3D MICROFLUIDIC CELL CULTURE DEVICE
Title (en)
METHODS FOR EVALUATING TUMOR CELL SPHEROIDS USING 3D MICROFLUIDIC CELL CULTURE DEVICE
Title (de)
VERFAHREN ZUR BEURTEILUNG VON TUMORZELLSPHÄROIDEN UNTER VERWENDUNG EINER DREIDIMENSIONALEN MIKROFLUIDISCHEN ZELLKULTURVORRICHTUNG
Title (fr)
PROCÉDÉS D'ÉVALUATION DE SPHÉROÏDES DE CELLULES TUMORALES À L'AIDE D'UN DISPOSITIF DE CULTURE CELLULAIRE MICROFLUIDIQUE 3D
Publication
Application
Priority
- US 201762480192 P 20170331
- US 2018025390 W 20180330
Abstract (en)
[origin: WO2018183839A1] Provided herein are methods for evaluating tumor cell spheroids in a three-dimensional microfluidic device by determining changes in the relative levels of live cells and dead cells in aliquots cultured under different conditions. Methods described herein allow ex vivo recapitulation of the tumor microenvironment such that the in vivo effectiveness of a test compound in treating tumor tissue may be predicted.
IPC 8 full level
A61K 39/395 (2006.01); A61K 49/00 (2006.01); A61K 49/04 (2006.01); A61K 49/06 (2006.01); A61K 49/16 (2006.01); A61K 49/18 (2006.01)
CPC (source: EP KR US)
C12M 23/16 (2013.01 - EP KR US); C12N 1/04 (2013.01 - US); C12N 9/6491 (2013.01 - EP US); C12Q 1/6869 (2013.01 - US); C12Q 1/6881 (2013.01 - US); C12Q 1/6886 (2013.01 - US); G01N 33/5011 (2013.01 - KR); G01N 33/5082 (2013.01 - EP KR US); G01N 33/582 (2013.01 - EP KR US); C12Y 304/24007 (2013.01 - EP US)
Citation (search report)
- [Y] WO 2016112172 A1 20160714 - DANA FARBER CANCER INST INC [US]
- [Y] US 2009081639 A1 20090326 - HILL PHIL [GB], et al
- [Y] US 2010111768 A1 20100506 - BANERJEE SAIBAL [US], et al
- [A] PERFETTO S P ET AL: "Amine reactive dyes: An effective tool to discriminate live and dead cells in polychromatic flow cytometry", JOURNAL OF IMMUNOLOGICAL METHODS, ELSEVIER SCIENCE PUBLISHERS B.V.,AMSTERDAM, NL, vol. 313, no. 1-2, 30 June 2006 (2006-06-30), pages 199 - 208, XP028017528, ISSN: 0022-1759, [retrieved on 20060630], DOI: 10.1016/J.JIM.2006.04.007
- [A] P. SABHACHANDANI ET AL: "Generation and functional assessment of 3D multicellular spheroids in droplet based microfluidics platform", LAB ON A CHIP, vol. 16, no. 3, 1 January 2016 (2016-01-01), pages 497 - 505, XP055552453, ISSN: 1473-0197, DOI: 10.1039/C5LC01139F
- See references of WO 2018183839A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
WO 2018183839 A1 20181004; WO 2018183839 A8 20181108; AU 2018243568 A1 20190919; CA 3055767 A1 20181004; CN 110505881 A 20191126; EP 3600425 A1 20200205; EP 3600425 A4 20201223; JP 2020515260 A 20200528; JP 7284095 B2 20230530; KR 102579906 B1 20230919; KR 20190130627 A 20191122; SG 11201908240Y A 20191030; US 11098369 B2 20210824; US 11572590 B2 20230207; US 2019112666 A1 20190418; US 2021087635 A1 20210325; US 2021363595 A1 20211125
DOCDB simple family (application)
US 2018025390 W 20180330; AU 2018243568 A 20180330; CA 3055767 A 20180330; CN 201880021776 A 20180330; EP 18775414 A 20180330; JP 2019553344 A 20180330; KR 20197031858 A 20180330; SG 11201908240Y A 20180330; US 201815999230 A 20180817; US 201816498907 A 20180330; US 202117379918 A 20210719