EP 3625333 A1 20200325 - PYRUVATE CARBOXYLASE HAVING A FEEDBACK-RESISTANT MUTATION, ENCODING DNA, PLASMIDES, AND MICROORGANISM FOR THE PRODUCTION THEREOF, AND METHODS FOR THE PRODUCTION OF PRODUCTS THE BIOSYNTHESIS OF WHICH INCLUDES OXALOACETATE AS PRECURSOR
Title (en)
PYRUVATE CARBOXYLASE HAVING A FEEDBACK-RESISTANT MUTATION, ENCODING DNA, PLASMIDES, AND MICROORGANISM FOR THE PRODUCTION THEREOF, AND METHODS FOR THE PRODUCTION OF PRODUCTS THE BIOSYNTHESIS OF WHICH INCLUDES OXALOACETATE AS PRECURSOR
Title (de)
PYRUVATCARBOXYLASE MIT EINER FEEDBACK-RESISTENZ VERURSACHENDE MUTATION, KODIERENDE DNA, PLASMIDE, SOWIE MIGROORGANISMUS ZUR PRODUKTION UND VERFAHREN ZUR HERSTELLUNG VON PRODUKTEN, DEREN BIOSYNTHESE OXALACETAT ALS VORSTUFE BEINHALTET
Title (fr)
PYRUVATE CARBOXYLASE PRÉSENTANT UNE MUTATION À L'ORIGINE D'UNE RÉSISTANCE DE RÉTRACTION, ADN CODANT, PLASMIDES, ET MICRO-ORGANISME DE PRODUCTION ET PROCÉDÉ DE PRÉPARATION DE PRODUITS DONT LA BIOSYNTHÈSE COMPREND L'OXALACÉTATE COMME PRÉCURSEUR
Publication
Application
Priority
- DE 102017004750 A 20170518
- DE 2018000107 W 20180417
Abstract (en)
[origin: WO2018210359A1] The invention relates to pyruvate carboxylase and a pyruvate carboxylase-encoding DNA, a plasmid containing said DNA and a microorganism for the production thereof, and to methods for the production of products the biosynthesis of which includes oxaloacetate as a precursor, and to a chromosome. The invention makes a DNA available that codes for a pyruvate carboxylase having an at least 70% sequence identity with sequence no. 1 and where the triplet that codes for isoleucine-1012 is replaced by a serine-encoding triplet in positions 3034-3036. The expression of said gene results in a pyruvate carboxylase by means of which the yield of the reaction of metabolic products derived from oxaloacetate is by 7% higher with respect to the strain containing the native Pyc.
IPC 8 full level
C12N 9/00 (2006.01); C12N 15/63 (2006.01); C12P 13/00 (2006.01)
CPC (source: EP)
C12N 9/93 (2013.01); C12N 15/63 (2013.01); C12P 13/04 (2013.01); C12P 13/06 (2013.01); C12P 13/08 (2013.01); C12P 13/20 (2013.01); C12Y 401/01001 (2013.01)
Citation (search report)
See references of WO 2018210359A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
WO 2018210359 A1 20181122; DE 102017004750 A1 20181122; EP 3625333 A1 20200325
DOCDB simple family (application)
DE 2018000107 W 20180417; DE 102017004750 A 20170518; EP 18723673 A 20180417