Global Patent Index - EP 3655546 A4

EP 3655546 A4 20210407 - TUMOR DETECTION AND MONITORING

Title (en)

TUMOR DETECTION AND MONITORING

Title (de)

TUMORDETEKTION UND -ÜBERWACHUNG

Title (fr)

DÉTECTION ET SURVEILLANCE DE TUMEUR

Publication

EP 3655546 A4 20210407 (EN)

Application

EP 18918562 A 20181012

Priority

  • US 201862672217 P 20180516
  • US 201816018926 A 20180626
  • US 2018055648 W 20181012

Abstract (en)

[origin: WO2019221769A1] The invention provides methods for detecting epigenetic changes, including but not limited to methylation changes, directly from biological samples, without the need for certain complex sample preparation steps. The invention provides Cas protein/guide RNA complexes that may be introduced directly into the sample, where the complexes target and bind the target region. The target region is thus enriched and isolated in a sequence- specific manner. The target region may then be subject to any suitable signal amplification assay to detect the epigenetic change in the target region. Detection of DNA hypermethylation in the target region is indicative of disease, such as cancer.

IPC 8 full level

C12Q 1/68 (2018.01); C12Q 1/70 (2006.01)

CPC (source: EP)

C12N 9/22 (2013.01); C12Q 1/6804 (2013.01); C12Q 1/6827 (2013.01)

C-Set (source: EP)

  1. C12Q 1/6827 + C12Q 2521/301 + C12Q 2521/319 + C12Q 2537/164
  2. C12Q 1/6804 + C12Q 2521/301 + C12Q 2521/319 + C12Q 2537/164
  3. C12Q 1/6804 + C12Q 2521/301 + C12Q 2521/319 + C12Q 2527/146 + C12Q 2537/164 + C12Q 2563/149

Citation (search report)

  • [Y] US 2016017396 A1 20160121 - CANN GORDON M [US], et al
  • [Y] SUTHEE RAPISUWON ET AL: "Circulating biomarkers to monitor cancer progression and treatment", COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL, vol. 14, 1 January 2016 (2016-01-01), Sweden, pages 211 - 222, XP055407446, ISSN: 2001-0370, DOI: 10.1016/j.csbj.2016.05.004
  • [Y] JONATHAN S. GOOTENBERG ET AL: "Nucleic acid detection with CRISPR-Cas13a/C2c2", SCIENCE, vol. 356, no. 6336, 28 April 2017 (2017-04-28), US, pages 438 - 442, XP055481345, ISSN: 0036-8075, DOI: 10.1126/science.aam9321
  • [Y] VON BUBNOFF NIKOLAS: "Liquid Biopsy: Approaches to Dynamic Genotyping in Cancer", ONCOLOGY RESEARCH AND TREATMENT, vol. 40, no. 7-8, 1 January 2017 (2017-01-01), CH, pages 409 - 416, XP055780028, ISSN: 2296-5270, Retrieved from the Internet <URL:https://www.karger.com/Article/Pdf/478864> DOI: 10.1159/000478864
  • [A] HEITZER ELLEN ET AL: "The potential of liquid biopsies for the early detection of cancer", NPJ PRECISION ONCOLOGY, vol. 1, no. 1, 17 October 2017 (2017-10-17), XP055775774, Retrieved from the Internet <URL:http://www.nature.com/articles/s41698-017-0039-5> DOI: 10.1038/s41698-017-0039-5
  • See references of WO 2019221773A1

Designated contracting state (EPC)

AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

Designated extension state (EPC)

BA ME

DOCDB simple family (publication)

WO 2019221769 A1 20191121; CA 3069939 A1 20191121; CA 3100492 A1 20191121; EP 3655546 A1 20200527; EP 3655546 A4 20210407; EP 3794139 A1 20210324; EP 3794139 A4 20220601; WO 2019221773 A1 20191121

DOCDB simple family (application)

US 2018054188 W 20181003; CA 3069939 A 20181012; CA 3100492 A 20181003; EP 18918562 A 20181012; EP 18919343 A 20181003; US 2018055648 W 20181012