EP 3708663 A1 20200916 - NUCLEASE-MEDIATED DNA ASSEMBLY

Title (en)

NUCLEASE-MEDIATED DNA ASSEMBLY

Title (de)

NUKLEASE-VERMITTELTE DNA VERKNÜPFUNG

Title (fr)

ASSEMBLAGE D'ADN MEDIÉE PAR NUCLEASE

Publication

EP 3708663 A1 20200916 (EN)

Application

EP 19218837 A 20150623

Priority

US 201462015809 P 20140623
US 201462016400 P 20140624
US 201462036983 P 20140813
EP 18162656 A 20150623
EP 15735807 A 20150623
US 2015037199 W 20150623

Abstract (en)

Methods are provided herein for assembling at least two nucleic acids using a sequence specific nuclease agent (e.g., a gRNA-Cas complex) to create end sequences having complementarity and subsequently assembling the overlapping complementary sequences. The nuclease agent (e.g., a gRNA-Cas complex) can create double strand breaks in dsDNA in order to create overlapping end sequences or can create nicks on each strand to produce complementary overhanging end sequences. Assembly using the method described herein can assemble any nucleic acids having overlapping sequences or can use a joiner oligo to assemble sequences without complementary ends.

IPC 8 full level

C12N 15/10 (2006.01); C12N 15/64 (2006.01); C12N 15/66 (2006.01)

CPC (source: EP IL KR RU US)

C12N 15/10 (2013.01 - RU); C12N 15/1031 (2013.01 - EP IL KR US); C12N 15/64 (2013.01 - EP IL KR RU US); C12N 15/66 (2013.01 - EP IL KR US)

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Designated contracting state (EPC)

AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

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