EP 3732485 A1 20201104 - PREDICTING THE METABOLIC CONDITION OF A CELL CULTURE
Title (en)
PREDICTING THE METABOLIC CONDITION OF A CELL CULTURE
Title (de)
VORHERSAGE DES METABOLISCHEN ZUSTANDS EINER ZELLKULTUR
Title (fr)
PRÉDICTION DE L'ETAT MÉTABOLIQUE D'UNE CULTURE CELLULAIRE
Publication
Application
Priority
- EP 17211217 A 20171229
- EP 2019050006 W 20190102
Abstract (en)
[origin: WO2019129891A1] The invention relates to a method for predicting the metabolic condition of a cell culture of cells of a specific cell type. The method comprises providing (102) a metabolic model (402) of a cell of the specific cell type and carrying out the following steps at each of a plurality of points in time during the cultivation of the cell culture: receiving (106) measured concentrations of several extracellular metabolites and a measured cell density in the culture medium; inputting (108) the received measured values as input parameter values into a trained machine learning program logic – MLP (218); predicting (110) extracellular flows (408) of the extracellular metabolites at a future point in time by means of the MLP; carrying out (112) a metabolic flow analysis for calculating the intracellular flows at the future point in time on the basis of the predicted extracellular flows and the stoichiometric equations of the metabolic model.
IPC 8 full level
G01N 33/50 (2006.01)
CPC (source: EP US)
C12M 41/38 (2013.01 - US); C12M 41/46 (2013.01 - US); C12N 5/0018 (2013.01 - US); G01N 33/5005 (2013.01 - EP); G06N 3/02 (2013.01 - US); G06N 20/00 (2019.01 - US); G16B 5/00 (2019.02 - US); G16B 5/30 (2019.02 - EP); G16B 40/20 (2019.02 - EP)
Citation (examination)
WO 2015092650 A1 20150625 - GLAXOSMITHKLINE BIOLOG SA [BE]
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
WO 2019129891 A1 20190704; CN 112119306 A 20201222; CN 112119306 B 20240903; EP 3732485 A1 20201104; JP 2021508872 A 20210311; JP 7092879 B2 20220628; US 2020377844 A1 20201203; US 2023313113 A1 20231005
DOCDB simple family (application)
EP 2019050006 W 20190102; CN 201980015112 A 20190102; EP 19700004 A 20190102; JP 2020534849 A 20190102; US 202016907786 A 20200622; US 202318331411 A 20230608