Global Patent Index - EP 3755726 A4

EP 3755726 A4 20220720 - FUSION PROTEINS FOR BASE EDITING

Title (en)

FUSION PROTEINS FOR BASE EDITING

Title (de)

FUSIONSPROTEINE ZUR BASISBEARBEITUNG

Title (fr)

PROTÉINES DE FUSION POUR ÉDITION DE BASE

Publication

EP 3755726 A4 20220720 (EN)

Application

EP 19757302 A 20190222

Priority

  • CN 2018076991 W 20180223
  • CN 2018100411 W 20180814
  • CN 2019075897 W 20190222

Abstract (en)

[origin: WO2019161783A1] Provided are fusion proteins that include an apolipoprotein B mRNA editing enzyme catalytic subunit 3A (APOBEC3A) and a clustered regularly interspaced short palindromic repeats (CRISPR) -associated (Cas) protein, optionally further with uracil glycosylase inhibitor (UGI). Such a fusion protein is able to conduct base editing in DNA by deaminating cytosine to uracil, even when the cytosine is in a GpC context or is methylated.

IPC 8 full level

C07K 19/00 (2006.01); C12N 9/22 (2006.01); C12N 15/11 (2006.01); C12N 15/62 (2006.01)

CPC (source: EP US)

C12N 9/22 (2013.01 - EP US); C12N 9/78 (2013.01 - EP US); C12N 15/01 (2013.01 - US); C12N 15/102 (2013.01 - EP); C12N 15/11 (2013.01 - US); C12N 15/62 (2013.01 - EP); C12N 15/907 (2013.01 - US); C12Y 305/04 (2013.01 - EP US); C07K 2319/00 (2013.01 - EP US); C12N 2310/20 (2017.04 - US); C12N 2800/80 (2013.01 - US)

Citation (search report)

  • [XI] WO 2017070632 A2 20170427 - HARVARD COLLEGE [US]
  • [T] WANG XIAO ET AL: "Cas12a Base Editors Induce Efficient and Specific Editing with Low DNA Damage Response", CELL REPORTS, vol. 31, no. 9, 1 June 2020 (2020-06-01), US, pages 107723, XP055859533, ISSN: 2211-1247, DOI: 10.1016/j.celrep.2020.107723
  • [Y] M. MITRA ET AL: "Structural determinants of human APOBEC3A enzymatic and nucleic acid binding properties", NUCLEIC ACIDS RESEARCH, vol. 42, no. 2, 1 January 2014 (2014-01-01), GB, pages 1095 - 1110, XP055322746, ISSN: 0305-1048, DOI: 10.1093/nar/gkt945
  • [Y] ERIC C. LOGUE ET AL: "A DNA Sequence Recognition Loop on APOBEC3A Controls Substrate Specificity", PLOS ONE, vol. 9, no. 5, 14 May 2014 (2014-05-14), pages e97062, XP055765496, DOI: 10.1371/journal.pone.0097062
  • [Y] KE SHI ET AL: "Structural basis for targeted DNA cytosine deamination and mutagenesis by APOBEC3A and APOBEC3B", NATURE STRUCTURAL & MOLECULAR BIOLOGY, vol. 24, no. 2, 1 February 2017 (2017-02-01), New York, pages 131 - 139, XP055753346, ISSN: 1545-9993, DOI: 10.1038/nsmb.3344
  • [Y] TAKAHIDE KOUNO ET AL: "Crystal structure of APOBEC3A bound to single-stranded DNA reveals structural basis for cytidine deamination and specificity", NATURE COMMUNICATIONS, vol. 8, no. 1, 1 April 2017 (2017-04-01), XP055605538, DOI: 10.1038/ncomms15024
  • See references of WO 2019161783A1

Designated contracting state (EPC)

AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

DOCDB simple family (publication)

WO 2019161783 A1 20190829; CN 111788232 A 20201016; EP 3755726 A1 20201230; EP 3755726 A4 20220720; US 11884947 B2 20240130; US 2021163913 A1 20210603; US 2024117335 A1 20240411

DOCDB simple family (application)

CN 2019075897 W 20190222; CN 201980015104 A 20190222; EP 19757302 A 20190222; US 201916770572 A 20190222; US 202318525555 A 20231130