EP 3759246 A1 20210106 - METHOD FOR THE AMPLIFICATION OF A NUCLEIC ACID WITH IMPROVED SPECIFICITY
Title (en)
METHOD FOR THE AMPLIFICATION OF A NUCLEIC ACID WITH IMPROVED SPECIFICITY
Title (de)
VERFAHREN ZUR AMPLIFIKATION EINER NUKLEINSÄURE MIT VERBESSERTER SPEZIFITÄT
Title (fr)
PROCÉDÉ D'AMPLIFICATION D'UN ACIDE NUCLÉIQUE À SPÉCIFICITÉ AMÉLIORÉE
Publication
Application
Priority
- EP 18159155 A 20180228
- EP 2019055060 W 20190228
Abstract (en)
[origin: WO2019166586A1] The invention relates to a method for the amplification of a nucleic acid using a nucleic acid polymerase via two primers which each have a sequence segment which binds to the target sequence and a sequence segment which does not bind thereto, wherein the second cannot serve as template for the polymerase, and two controller oligonucleotides, which are each complementary with the primers and a part of the sequence segment synthesized therefrom, and serve in the detachment of the synthesized strand from the template. The controllers also comprise modified nucleotide building blocks such that they cannot serve as template for the activity of the first template-dependent nucleic acid polymerase.
IPC 8 full level
C12Q 1/6848 (2018.01)
CPC (source: EP US)
C12Q 1/6848 (2013.01 - EP US); C12Q 1/686 (2013.01 - US)
Citation (search report)
See references of WO 2019166586A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
WO 2019166586 A1 20190906; EP 3759246 A1 20210106; US 2021071244 A1 20210311
DOCDB simple family (application)
EP 2019055060 W 20190228; EP 19710329 A 20190228; US 201916976128 A 20190228