EP 3788155 A1 20210310 - LENTIVIRAL-BASED VECTORS AND RELATED SYSTEMS AND METHODS FOR EUKARYOTIC GENE EDITING
Title (en)
LENTIVIRAL-BASED VECTORS AND RELATED SYSTEMS AND METHODS FOR EUKARYOTIC GENE EDITING
Title (de)
LENTIVIRENBASIERTE VEKTOREN UND VERWANDTE SYSTEME UND VERFAHREN FÜR EUKARYOTISCHE GENEDITIERUNG
Title (fr)
VECTEURS LENTIVIRAUX AINSI QUE SYSTÈMES ET PROCÉDÉS ASSOCIÉS POUR L'ÉDITION DE GÈNES EUCARYOTES
Publication
Application
Priority
- US 201862665080 P 20180501
- US 2019030198 W 20190501
Abstract (en)
[origin: WO2019213257A1] Provided are compositions, systems, and methods useful for effecting gene editing in eukaryotic cells. Compositions include plasmids that encode one or more viral fusion proteins in which one or more viral proteins are fused with an aptamer-binding protein. Compositions also include plasmids that encode a non-viral nucleic acid sequence, wherein the non-viral nucleic acid sequence encodes a CRISPR system component. In some instances, the non-viral nucleic acid sequence also includes an aptamer sequence. The plasmids can be used to generate viral particles, including lentivirus-like particles that contain a viral fusion protein and a non-viral RNA sequence. Systems of producing such viral particles are provided. Also provided are methods of using the viral particles of the disclosure to effect gene editing in eukaryotic cells.
IPC 8 full level
C12N 15/86 (2006.01); C07K 14/005 (2006.01); C12N 15/10 (2006.01); C12N 15/115 (2010.01)
CPC (source: EP US)
C07K 14/005 (2013.01 - EP); C07K 14/155 (2013.01 - US); C12N 9/22 (2013.01 - US); C12N 15/113 (2013.01 - EP US); C12N 15/115 (2013.01 - EP); C12N 15/86 (2013.01 - EP US); C07K 2319/735 (2013.01 - EP US); C12N 2310/16 (2013.01 - EP US); C12N 2310/20 (2017.05 - EP US); C12N 2310/3519 (2013.01 - EP); C12N 2320/32 (2013.01 - EP); C12N 2740/15022 (2013.01 - US); C12N 2740/16022 (2013.01 - EP); C12N 2740/16023 (2013.01 - EP); C12N 2740/16043 (2013.01 - EP); C12N 2740/16052 (2013.01 - EP); C12N 2740/16222 (2013.01 - EP); C12N 2795/10122 (2013.01 - EP); C12N 2795/10322 (2013.01 - EP); C12N 2795/18122 (2013.01 - EP)
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
WO 2019213257 A1 20191107; AU 2019262029 A1 20201119; CA 3098137 A1 20191107; EP 3788155 A1 20210310; JP 2021522783 A 20210902; JP 7506405 B2 20240626; US 2021047375 A1 20210218
DOCDB simple family (application)
US 2019030198 W 20190501; AU 2019262029 A 20190501; CA 3098137 A 20190501; EP 19727544 A 20190501; JP 2020560827 A 20190501; US 201917051296 A 20190501