EP 3790982 A1 20210317 - IN SITU CELL ANALYSIS IN CELL CULTURE SYSTEM
Title (en)
IN SITU CELL ANALYSIS IN CELL CULTURE SYSTEM
Title (de)
IN-SITU ZELLANALYSE IM ZELLKULTURSYSTEM
Title (fr)
ANALYSE CELLULAIRE IN SITU DANS UN SYSTÈME DE CULTURE CELLULAIRE
Publication
Application
Priority
- AT 503882018 A 20180509
- EP 2019061606 W 20190507
Abstract (en)
[origin: WO2019215112A1] The invention relates to an in situ method, including a) determining a molecule selected from the group consisting of cell surface molecules and extracellular matrix molecules in a two- or three-dimensional cell culture system containing living cells and cell culture medium, having the steps of i) providing an analyte probe consisting of a detection element, which binds the molecule, and one or more identification elements; ii) binding the analyte probe to the molecule in the cell culture system, wherein the growth ability of the contained living cells is not substantially impaired by said step; iii) optionally removing unbound analyte probes; iv) releasing the analyte probe; v) transferring the analyte probe into a container which differs from the cell culture system; and vi) detecting the identification element(s); and b) continuing the cell cultivation in the cell culture system.
IPC 8 full level
C12Q 1/02 (2006.01); C12Q 1/6823 (2018.01); G01N 33/537 (2006.01); G01N 33/543 (2006.01)
CPC (source: AT EP US)
C12N 15/115 (2013.01 - AT US); C12Q 1/02 (2013.01 - AT EP); C12Q 1/68 (2013.01 - AT); C12Q 1/6804 (2013.01 - EP US); C12Q 1/6841 (2013.01 - EP US); G01N 33/53 (2013.01 - AT); G01N 33/537 (2013.01 - EP); G01N 33/5375 (2013.01 - EP); G01N 33/54306 (2013.01 - EP); C12Q 2525/161 (2013.01 - US); C12Q 2525/205 (2013.01 - US)
Citation (search report)
See references of WO 2019215112A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
WO 2019215112 A1 20191114; AT 521238 A1 20191115; AT 521238 B1 20200215; CN 112513286 A 20210316; CN 112513286 B 20220726; EP 3790982 A1 20210317; US 11279971 B2 20220322; US 2021071241 A1 20210311
DOCDB simple family (application)
EP 2019061606 W 20190507; AT 503882018 A 20180509; CN 201980045951 A 20190507; EP 19727591 A 20190507; US 201917053686 A 20190507