EP 3814534 A4 20220504 - METHODS AND COMPOSITIONS FOR IMPROVED MULTIPLEX GENOTYPING AND SEQUENCING
Title (en)
METHODS AND COMPOSITIONS FOR IMPROVED MULTIPLEX GENOTYPING AND SEQUENCING
Title (de)
VERFAHREN UND ZUSAMMENSETZUNGEN ZUR VERBESSERTEN MULTIPLEXGENOTYPISIERUNG UND -SEQUENZIERUNG
Title (fr)
PROCÉDÉS ET COMPOSITIONS POUR GÉNOTYPAGE ET SÉQUENÇAGE MULTIPLEXES AMÉLIORÉS
Publication
Application
Priority
- US 201862692293 P 20180629
- US 2019039921 W 20190628
Abstract (en)
[origin: WO2020006475A1] The technology described herein is directed to methods of designing primers for multiplex PCR amplification. Also described herein are methods for equalization of reads in these approaches. A variation is described herein that permits single base multiplexed sequencing on an NGS platform. Also described herein are methods to rapidly analyze NGS sequencing data to automatically provide genotype or sequencing results and methods to identify and quantify low abundance rare variants in clinically relevant genes in a minority of tumor cells from a complex mixture of cells.
IPC 8 full level
G16B 25/20 (2019.01); G16B 40/30 (2019.01); C12Q 1/686 (2018.01)
CPC (source: EP US)
C12Q 1/686 (2013.01 - US); G16B 25/20 (2019.01 - EP US); G16B 40/00 (2019.01 - US); G16B 40/30 (2019.01 - EP); C12Q 1/686 (2013.01 - EP)
Citation (search report)
- [IA] US 2016068903 A1 20160310 - ZHOU XIAOCHUAN [US], et al
- [IA] L. KAPLINSKI ET AL: "MultiPLX: automatic grouping and evaluation of PCR primers", BIOINFORMATICS, vol. 21, no. 8, 14 December 2004 (2004-12-14), GB, pages 1701 - 1702, XP055227877, ISSN: 1367-4803, DOI: 10.1093/bioinformatics/bti219
- [IA] HUANG Y.-C. ET AL: "Integrated minimum-set primers and unique probe design algorithms for differential detection on symptom-related pathogens", BIOINFORMATICS, vol. 21, no. 24, 15 December 2005 (2005-12-15), GB, pages 4330 - 4337, XP055904050, ISSN: 1367-4803, Retrieved from the Internet <URL:https://watermark.silverchair.com/bti730.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAAvUwggLxBgkqhkiG9w0BBwagggLiMIIC3gIBADCCAtcGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQM-nvzleHawCLxsndrAgEQgIICqMfznV835IfQNbw8xekf8JtS26Wmoctxg9wi5js3YtVbYkDFwL6RS3DpMjameqqdMFxf5IisjwIpaj0TuQ57iUcmYFcsu> DOI: 10.1093/bioinformatics/bti730
- See references of WO 2020006475A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
WO 2020006475 A1 20200102; CA 3103811 A1 20200102; EP 3814534 A1 20210505; EP 3814534 A4 20220504; US 2022310203 A1 20220929
DOCDB simple family (application)
US 2019039921 W 20190628; CA 3103811 A 20190628; EP 19824681 A 20190628; US 201917255722 A 20190628