EP 3830255 A1 20210609 - NON-SPECIFIC NUCLEASES FROM THE GENUS PSEUDOMONAS
Title (en)
NON-SPECIFIC NUCLEASES FROM THE GENUS PSEUDOMONAS
Title (de)
NICHTSPEZIFISCHE NUKLEASEN AUS DER GATTUNG PSEUDOMONAS
Title (fr)
NUCLÉASES NON SPÉCIFIQUES ISSUES DU GENRE PSEUDOMONAS
Publication
Application
Priority
- EP 18186232 A 20180730
- EP 2019070148 W 20190726
Abstract (en)
[origin: WO2020025468A1] The present invention provides a composition comprising an isolated polypeptide having non-specific nuclease (NSN) activity for degrading nucleic acids comprising or consisting of at least 70% amino acid sequence identity to SEQ ID NO:2, wherein said polypeptide has NSN activity in a solution of about 4°C, and a cation complexing agent such as EDTA. Said composition may have preferentially a neutral to basic pH. A kit comprising said composition and a method using said composition are also disclosed.
IPC 8 full level
C12N 9/22 (2006.01); C07K 14/21 (2006.01)
CPC (source: EP US)
C12N 9/22 (2013.01 - EP US); C12N 9/96 (2013.01 - US); G01N 1/4044 (2013.01 - US)
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
WO 2020025468 A1 20200206; CN 112424350 A 20210226; EP 3830255 A1 20210609; US 11999980 B2 20240604; US 2021301273 A1 20210930
DOCDB simple family (application)
EP 2019070148 W 20190726; CN 201980047581 A 20190726; EP 19742613 A 20190726; US 201917264748 A 20190726