EP 3851853 A1 20210721 - METHOD FOR SEPARATION OF SPERM WITH UNDAMAGED INTACT HEADS FROM SPERM WITH DAMAGED HEADS AND SOMATIC CELLS
Title (en)
METHOD FOR SEPARATION OF SPERM WITH UNDAMAGED INTACT HEADS FROM SPERM WITH DAMAGED HEADS AND SOMATIC CELLS
Title (de)
VERFAHREN ZUR TRENNUNG VON SPERMIEN MIT UNBESCHÄDIGTEM INTAKTEM KOPF VON SPERMIEN MIT BESCHÄDIGTEM KOPF UND SOMATISCHEN ZELLEN
Title (fr)
PROCÉDÉ DE SÉPARATION DES SPERMATOZOÏDES DONT LA TÊTE EST INTACTE ET NON ENDOMMAGÉE DES SPERMATOZOÏDES DONT LA TÊTE EST ENDOMMAGÉE ET DES CELLULES SOMATIQUES
Publication
Application
Priority
EP 20152484 A 20200117
Abstract (en)
The present invention provides a method of separation of sperm cells with undamaged intact heads from sperm cells with damaged heads and/or somatic cells and/or cell debris in a cell sample obtained from a human, comprising the steps of:- providing an anti-CD46 antibody bound to a carrier and/or to a tag;- contacting the cell sample with the anti-CD46 antibody bound to the carrier and/or to the tag in order to bind CD46 presenting cells and/or cell debris from the cell sample to the anti-CD46 antibody;- removing the CD46 presenting cells bound via the anti-CD46 antibody to the carrier and/or to the tag to obtain a cell sample free of CD46 presenting cells, wherein CD46 presenting cells include sperm cells with damaged heads, somatic cells and cell debris.The technology of the invention is in particular designed to separate sperm with undamaged intact heads to be further used in assisted reproduction, human medicine and research.
IPC 8 full level
G01N 33/50 (2006.01); C12N 5/076 (2010.01)
CPC (source: CZ EP US)
B01D 57/00 (2013.01 - CZ); C07K 16/2896 (2013.01 - EP US); C12N 5/061 (2013.01 - CZ); C12N 5/0612 (2013.01 - EP US); G01N 33/5091 (2013.01 - EP)
Citation (applicant)
US 2017191029 A1 20170706 - EDWARDS J LANNETT [US], et al
Citation (search report)
- [Y] WO 2017120173 A1 20170713 - UNIV TENNESSEE RES FOUND [US]
- [Y] US 2008004268 A1 20080103 - NGUYEN DUY [DE], et al
- [Y] SILVA P F N ET AL: "Detection of damage in mammalian sperm cells", THERIOGENOLOGY, LOS ALTOS, CA, US, vol. 65, no. 5, 15 March 2006 (2006-03-15), pages 958 - 978, XP027930357, ISSN: 0093-691X, [retrieved on 20060315]
- [Y] MIZUNO ET AL: "CD55 in rat male reproductive tissue: Differential expression in testis and expression of a unique truncated isoform on spermatozoa", MOLECULAR IMMUNOLOGY, PERGAMON, GB, vol. 44, no. 7, 26 November 2006 (2006-11-26), pages 1613 - 1622, XP005792693, ISSN: 0161-5890, DOI: 10.1016/J.MOLIMM.2006.08.018
- [Y] D'CRUZ O J ET AL: "SPERMICIDAL ACTIVITY OF OXOVANADIUM(IV) COMPLEXES OF 1,10-PHENANTHROLINE, 2,2'-BIPYRIDYL, 5'-BROMO-2'-HYDROXYACETOPHENONE AND DERIVATIVES IN HUMANS", BIOLOGY OF REPRODUCTION, NEW YORK, NY [U.A.] : ACADEM. PRESS, US, vol. 60, no. 2, 1 January 1999 (1999-01-01), pages 435 - 444, XP000981488, ISSN: 0006-3363, DOI: 10.1095/BIOLREPROD60.2.435
- [A] MICHAELA FROLÍKOVÁ ET AL: "Role of complement regulatory proteins CD46, CD55 and CD59 in reproduction", FOLIA ZOOLOGICA, vol. 61, no. 1, 1 April 2012 (2012-04-01), Brno, CZ, pages 84 - 94, XP055712482, ISSN: 0139-7893, DOI: 10.25225/fozo.v61.i1.a12.2012
- [A] MICHAELA FROLIKOVA ET AL: "Characterization of CD46 and [beta]1 integrin dynamics during sperm acrosome reaction", SCIENTIFIC REPORTS, vol. 6, no. 1, 26 September 2016 (2016-09-26), XP055712101, DOI: 10.1038/srep33714
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
EP 3851853 A1 20210721; CZ 2020224 A3 20210721; CZ 308864 B6 20210721; EP 4090972 A1 20221123; US 2023044560 A1 20230209; WO 2021143959 A1 20210722
DOCDB simple family (application)
EP 20152484 A 20200117; CZ 2020224 A 20200417; CZ 2021050005 W 20210117; EP 21703813 A 20210117; US 202117792566 A 20210117