EP 3901163 A4 20221005 - BOTULINUM TOXIN PRODUCING METHOD
Title (en)
BOTULINUM TOXIN PRODUCING METHOD
Title (de)
VERFAHREN ZUR HERSTELLUNG VON BOTULINUMTOXIN
Title (fr)
PROCÉDÉ DE PRODUCTION DE TOXINE BOTULIQUE
Publication
Application
Priority
- JP 2018239214 A 20181221
- JP 2019049436 W 20191217
Abstract (en)
[origin: EP3901163A1] Provided is a botulinum toxin producing method which is simple achieves a high toxin yield, and obtains a toxin having high specific activity. This botulinum toxin producing method includes: (A) a step in which a botulinum toxin is produced from botulinum toxin-producing bacteria in a medium, and a mixture a is obtained which contains a botulinum toxin, a bacterial component, and a nucleic acid component derived from the botulinum toxin; (B) a step in which the mixture a is subjected to the removal of the bacterial component, and a mixture b is obtained which contains a nucleic acid component and a botulinum toxin; (C) a step in which an endonuclease is added to the mixture b and a mixture c is obtained which contains a nucleic acid degradation product and a botulinum toxin; and (D) a step in which the mixture c is subjected to removal of the nucleic acid degradation product, and an isolated botulinum toxin liquid d is obtained.
IPC 8 full level
C07K 14/195 (2006.01); C07K 1/36 (2006.01); C07K 14/33 (2006.01); C12N 9/22 (2006.01); C12N 9/52 (2006.01); C12P 21/02 (2006.01)
CPC (source: EP KR US)
C07K 14/33 (2013.01 - EP KR); C12N 9/22 (2013.01 - EP); C12N 9/52 (2013.01 - EP US); C12P 21/02 (2013.01 - EP); C12Y 304/24069 (2013.01 - EP US); Y02A 50/30 (2018.01 - EP)
Citation (search report)
- [XI] KR 101025617 B1 20110330
- [A] US 2011092682 A1 20110421 - RUEGG CURTIS L [US]
- [XI] KITAMURA M ET AL: "Purification and some properties of Clostridium botulinum type-E toxin", BBA - PROTEIN STRUCTURE, ELSEVIER SCIENCE BV, AMSTERDAM, NL, vol. 168, no. 2, 21 October 1968 (1968-10-21), pages 207 - 217, XP026773522, ISSN: 0005-2795, [retrieved on 19681021], DOI: 10.1016/0005-2795(68)90144-X
- See also references of WO 2020129986A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
EP 3901163 A1 20211027; EP 3901163 A4 20221005; CA 3124535 A1 20200625; CN 113056478 A 20210629; JP 7454507 B2 20240322; JP WO2020129986 A1 20211104; KR 20210106983 A 20210831; US 11926853 B2 20240312; US 2022081682 A1 20220317; WO 2020129986 A1 20200625
DOCDB simple family (application)
EP 19898205 A 20191217; CA 3124535 A 20191217; CN 201980076236 A 20191217; JP 2019049436 W 20191217; JP 2020561461 A 20191217; KR 20217013829 A 20191217; US 201917416342 A 20191217