EP 3927717 A4 20221221 - GUIDE STRAND LIBRARY CONSTRUCTION AND METHODS OF USE THEREOF
Title (en)
GUIDE STRAND LIBRARY CONSTRUCTION AND METHODS OF USE THEREOF
Title (de)
KONSTRUKTION EINER FÜHRUNGSSTRANGBIBLIOTHEK UND VERWENDUNGSVERFAHREN DAFÜR
Title (fr)
CONSTRUCTION DE BANQUES DE BRINS GUIDES ET PROCÉDÉS D'UTILISATION ASSOCIÉS
Publication
Application
Priority
- US 201962807679 P 20190219
- US 201962902601 P 20190919
- US 2020018701 W 20200218
Abstract (en)
[origin: WO2020172199A1] An improved method for the rapid and efficient production of guide strand libraries is disclosed. Also included are kits comprising reagents suitable for practicing the method. Embodiments may include a polynucleotide comprising a sequence encoding for a constant region comprising a protein binding segment of an RNA component of a CRISPR complex. The sequence may comprise a non-palindromic recognition sequence for a first restriction enzyme having methyltransferase activity and configured to cleave at least 17 nucleotides outside of the non-palindromic recognition sequence, wherein at least one base of the non-palindromic recognition sequence is configured to be methylated by the first restriction enzyme, thereby blocking endonuclease activity of the first restriction enzyme.
IPC 8 full level
C07H 21/00 (2006.01); C12N 15/10 (2006.01); C12N 15/11 (2006.01); C12P 19/34 (2006.01); C12Q 1/68 (2018.01); C12Q 1/6806 (2018.01)
CPC (source: EP)
C12N 15/10 (2013.01); C12N 15/102 (2013.01); C12N 15/111 (2013.01); C12P 19/34 (2013.01); C12Q 1/6806 (2013.01); C12N 2310/20 (2017.04); C12N 2330/31 (2013.01)
C-Set (source: EP)
Citation (search report)
- [XI] WO 2011133695 A2 20111027 - SWIFT BIOSCIENCES INC [US], et al
- [A] US 2018100147 A1 20180412 - YATES JOSHUA [US], et al
- [X] KARLSSON KASPER ET AL: "Amplification-free sequencing of cell-free DNA for prenatal non-invasive diagnosis of chromosomal aberrations", GENOMICS, vol. 105, no. 3, 24 December 2014 (2014-12-24), US, pages 150 - 158, XP055977655, ISSN: 0888-7543, DOI: 10.1016/j.ygeno.2014.12.005
- [AP] DON YATES JOSHUA: "A CRISPR/Cas9 Tissue Specific Forward Genetic Screening Method in Danio rerio Method in Danio rerio", 9 April 2019 (2019-04-09), XP055977331, Retrieved from the Internet <URL:https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=10282&context=etd> [retrieved on 20221102]
- See references of WO 2020172199A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
WO 2020172199 A1 20200827; EP 3927717 A1 20211229; EP 3927717 A4 20221221
DOCDB simple family (application)
US 2020018701 W 20200218; EP 20759378 A 20200218