EP 3929280 A1 20211229 - PROCESS FOR PRODUCING LIVER CELLS
Title (en)
PROCESS FOR PRODUCING LIVER CELLS
Title (de)
VERFAHREN ZUR HERSTELLUNG VON LEBERZELLEN
Title (fr)
PROCÉDÉ DE PRODUCTION DE CELLULES HÉPATIQUES
Publication
Application
Priority
EP 20182747 A 20200626
Abstract (en)
The present invention relates to a process for producing liver cells, especially liver stem cells, which after injection into a mammal or in vitro form liver cells that are differentiated, especially differentiated into hepatocytes, into cholangiocytes, and preferably also into liver sinusoidal endothelial cells (LSEC) that can e.g. form blood sinusoidal capillaries. The invention is based on in vitro producing liver stem cells from a sample of liver tissue, cultivating the liver stem cells in vitro for an increase in cell number. It has been found that the liver stem cells that are produced by the process of the invention can be cultivated and increased in number while maintaining their capability to differentiate into liver cells, especially into hepatocytes, cholangiocytes, and preferably also into LSEC. Accordingly, the process of the invention is suitable for producing liver stem cells that are autologous for the originator of the sample of liver tissue.
IPC 8 full level
C12N 5/071 (2010.01); A61K 35/407 (2015.01)
CPC (source: EP US)
A61K 35/407 (2013.01 - EP); C12N 5/0672 (2013.01 - EP US); C12N 2320/30 (2013.01 - US); C12N 2501/10 (2013.01 - EP); C12N 2501/11 (2013.01 - US); C12N 2503/02 (2013.01 - US); C12N 2533/54 (2013.01 - EP)
Citation (applicant)
- HERRERA ET AL., STEM CELLS, vol. 24, 2006, pages 2840 - 2850
- KIM ET AL., J. HEPATOL., vol. 70, 2019, pages 97 - 107
- CORBETTDUNCAN, FRONTIERS IN MEDICINE, vol. 6, 2019
Citation (search report)
- [X] WO 2007071339 A1 20070628 - UNIV CATHOLIQUE LOUVAIN [BE], et al
- [A] WO 2014150720 A1 20140925 - UNIV MICHIGAN [US]
- [X] JAUREGUI R G ET AL: "MOUSE LIVER REPOPULATION WITH HUMAN LIVER STEM CELLS", JOURNAL OF HEPATOLOGY, vol. 64, no. Suppl. 2, 2016, & INTERNATIONAL LIVER CONGRESS OF THE EUROPEAN-ASSOCIATION-FOR-THE-STUDY-OF-THE-LIVER (EASL); BARCELONA, SPAIN; APRIL 13 -17, 2016, pages S154 - S155, XP009524476
- [X] FEKETE-DRIMUSZ N ET AL: "298 ISOLATION AND CULTIVATION OF PURE HUMAN ADULT LIVER STEM CELL POPULATIONS FROM PATIENT LIVER RESECTATES", JOURNAL OF HEPATOLOGY, vol. 58, 1 January 2013 (2013-01-01), XP028588210, ISSN: 0168-8278, DOI: 10.1016/S0168-8278(13)60300-1
- [A] DAIHACHIRO TOMOTSUNE ET AL: "Enrichment of Pluripotent Stem Cell-Derived Hepatocyte-Like Cells by Ammonia Treatment", PLOS ONE, vol. 11, no. 9, 15 September 2016 (2016-09-15), pages 1 - 13, XP055687065, DOI: 10.1371/journal.pone.0162693
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
EP 3929280 A1 20211229; EP 4172317 A1 20230503; US 2023250398 A1 20230810; WO 2021259814 A1 20211230
DOCDB simple family (application)
EP 20182747 A 20200626; EP 2021066719 W 20210618; EP 21732343 A 20210618; US 202118010340 A 20210618