EP 3980552 A1 20220413 - NOVEL SYSTEMS, METHODS AND COMPOSITIONS FOR THE DIRECT SYNTHESIS OF STICKY ENDED POLYNUCLEOTIDES
Title (en)
NOVEL SYSTEMS, METHODS AND COMPOSITIONS FOR THE DIRECT SYNTHESIS OF STICKY ENDED POLYNUCLEOTIDES
Title (de)
NEUARTIGE SYSTEME, VERFAHREN UND ZUSAMMENSETZUNGEN ZUR DIREKTEN SYNTHESE VON POLYNUKLEOTIDEN MIT ÜBERHÄNGENDEN ENDEN
Title (fr)
NOUVEAUX SYSTÈMES, MÉTHODES ET COMPOSITIONS POUR LA SYNTHÈSE DIRECTE DE POLYNUCLÉOTIDES À EXTRÉMITÉS COLLANTES
Publication
Application
Priority
- US 201962858163 P 20190606
- US 2020036645 W 20200608
Abstract (en)
[origin: WO2020247927A1] The current inventive technology includes systems, methods, and compositions for directly synthesizing sticky ended DNA fragments for subsequent gene assembly. In a preferred embodiment, the inventive technology includes strategies for the direct synthesis of sticky ended DNA with 5' overhangs that have any desired length and base composition, using typical PCR protocols with no additional manipulation. In another embodiment, the inventive technology includes the direct synthesis of sticky ended DNA using chemically modified oligonucleotide primers in a polymerase chain reaction (PCR). In certain embodiments, the inventive technology allows for the generation of larger DNA constructs formed by the sticky-ended assemblies generally described herein compared to traditional synthesis and ligation applications.
IPC 8 full level
C07C 205/06 (2006.01); C07D 239/54 (2006.01); C07H 21/04 (2006.01); C12P 19/34 (2006.01)
CPC (source: EP US)
C12N 9/93 (2013.01 - US); C12N 15/1031 (2013.01 - EP); C12P 19/34 (2013.01 - EP US); C12Q 1/6853 (2013.01 - US); C12Q 1/686 (2013.01 - EP); C12Y 605/01001 (2013.01 - US)
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
WO 2020247927 A1 20201210; EP 3980552 A1 20220413; EP 3980552 A4 20230621; JP 2022534790 A 20220803; US 2022162687 A1 20220526
DOCDB simple family (application)
US 2020036645 W 20200608; EP 20818661 A 20200608; JP 2021571913 A 20200608; US 202017616958 A 20200608