EP 3988639 A4 20230726 - CELL RECOVERY METHOD AND CELL CULTURE DEVICE
Title (en)
CELL RECOVERY METHOD AND CELL CULTURE DEVICE
Title (de)
ZELLRÜCKGEWINNUNGSVERFAHREN UND ZELLKULTURVORRICHTUNG
Title (fr)
PROCÉDÉ DE RÉCUPÉRATION DE CELLULES ET DISPOSITIF DE CULTURE CELLULAIRE
Publication
Application
Priority
- JP 2019114230 A 20190620
- JP 2020023462 W 20200615
Abstract (en)
[origin: EP3988639A1] Damage to cells is suppressed in a cell recovery method that does not require a centrifuge. When recovering cells which are cultured in at least one container containing a liquid medium and adhere to an inner surface of the container, a medium discharge step (step S 11) of discharging the medium from the container, a peeling liquid supply step (step S12) of, after the medium is discharged, supplying a peeling liquid for peeling the cells from the inner surface of the container to the container, a peeling liquid discharge step (step S14) of discharging the peeling liquid from the container before the cells are completely peeled from the inner surface of the container, a waiting step (step S15) of, after the peeling liquid is discharged, waiting until the cells are peeled by action of a residual peeling liquid, and a recovery liquid supply step (step S16) of, after the waiting step is completed, supplying a recovery liquid for recovering the cells to the container are performed.
IPC 8 full level
C12M 1/32 (2006.01); C12M 3/00 (2006.01); C12N 1/02 (2006.01); C12N 5/00 (2006.01)
CPC (source: CN EP KR US)
C12M 3/06 (2013.01 - US); C12M 23/20 (2013.01 - CN); C12M 23/34 (2013.01 - CN); C12M 23/44 (2013.01 - US); C12M 27/02 (2013.01 - CN); C12M 29/06 (2013.01 - CN); C12M 33/00 (2013.01 - CN); C12M 41/24 (2013.01 - CN); C12M 41/34 (2013.01 - CN); C12M 47/02 (2013.01 - EP KR); C12M 47/04 (2013.01 - US); C12N 1/02 (2013.01 - EP); C12N 5/0606 (2013.01 - EP); C12N 5/0696 (2013.01 - EP); C12N 2509/00 (2013.01 - EP US)
Citation (search report)
- [I] JP 2017201946 A 20171116 - PANASONIC CORP
- [I] US 2017037357 A1 20170209 - CATTARUZZI GIACOMO [IT], et al
- [X] WO 2019097946 A1 20190523 - HITACHI LTD [JP]
- [I] JP 2016036274 A 20160322 - IHI CORP
- [I] JP 6303497 B2 20180404
- [I] WO 2018135289 A1 20180726 - TOYO SEIKAN GROUP HOLDINGS LTD [JP]
- [X] EP 3315604 A1 20180502 - KANEKA CORP [JP], et al
- [I] EP 3098300 A1 20161130 - NISSAN CHEMICAL IND LTD [JP], et al
- [X] MATHER, ROBERTS: "Introduction To Cell And Tissue Culture: Theory And Practice", 1998, PLENUM PRESS, New York, ISBN: 0-306-45859-4, article CHAPTER 5 - STANDARD CELL CULTURE TECHNIQUES: "Subculturing Adherent Cells", pages: 64 - 65, XP002809364
- See also references of WO 2020255930A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
EP 3988639 A1 20220427; EP 3988639 A4 20230726; CN 114026217 A 20220208; JP 2021000007 A 20210107; JP 7428866 B2 20240207; KR 20220024054 A 20220303; TW 202100740 A 20210101; US 2022356437 A1 20221110; WO 2020255930 A1 20201224
DOCDB simple family (application)
EP 20826608 A 20200615; CN 202080044142 A 20200615; JP 2019114230 A 20190620; JP 2020023462 W 20200615; KR 20217041093 A 20200615; TW 109116635 A 20200520; US 202017619895 A 20200615