Global Patent Index - EP 3990630 A1

EP 3990630 A1 20220504 - ELECTRONIC DETECTION OF A TARGET BASED ON ENZYMATIC CLEAVAGE OF A REPORTER MOIETY

Title (en)

ELECTRONIC DETECTION OF A TARGET BASED ON ENZYMATIC CLEAVAGE OF A REPORTER MOIETY

Title (de)

ELEKTRONISCHE DETEKTION EINES ZIELS AUF BASIS VON ENZYMATISCHER SPALTUNG EINER REPORTEREINHEIT

Title (fr)

DÉTECTION ÉLECTRONIQUE D'UNE CIBLE SUR LA BASE D'UN CLIVAGE ENZYMATIQUE D'UNE FRACTION RAPPORTEUR

Publication

EP 3990630 A1 20220504 (EN)

Application

EP 20831413 A 20200625

Priority

  • US 201916459298 A 20190701
  • US 202063036772 P 20200609
  • US 201962866312 P 20190625
  • US 2020039678 W 20200625

Abstract (en)

[origin: CA3144955A1] Apparatuses, systems, and methods are disclosed for target detection based on collateral cleavage of a reporter (730) by an enzyme (720). A biologically gated transistor (106) may include a channel (710) and a reporter moiety (730) immobilized to the channel (710). The state of the reporter moiety (730) affects output signals from the biologically gated transistor (106) when excitation conditions are applied to the biologically gated transistor (106) and a sample fluid (110) is applied in contact with the channel (710). A sample fluid (110) may include an enzyme (720) configured to activate in response to a target nucleic acid (722) to cleave the reporter moiety (730). Excitation circuitry (1502) applies the excitation conditions, and measurement circuitry (1504) measures output signals from the biologically gated transistor (106). An analysis module (116) determines a parameter relating to presence of the target nucleic acid (722), based on the one or more measurements.

IPC 8 full level

C12N 9/22 (2006.01); C12N 15/10 (2006.01); C12Q 1/68 (2018.01); C12Q 1/6811 (2018.01); C12Q 1/6825 (2018.01)

CPC (source: EP KR)

C12N 9/22 (2013.01 - EP KR); C12Q 1/6825 (2013.01 - EP KR); C12Q 2521/301 (2013.01 - KR)

Designated contracting state (EPC)

AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

Designated extension state (EPC)

BA ME

DOCDB simple family (publication)

CA 3144955 A1 20201230; CN 114341339 A 20220412; EP 3990630 A1 20220504; EP 3990630 A4 20220817; KR 20220024917 A 20220303

DOCDB simple family (application)

CA 3144955 A 20200625; CN 202080060246 A 20200625; EP 20831413 A 20200625; KR 20227002443 A 20200625