EP 4028509 A1 20220720 - ATP-BASED CELL SORTING AND HYPERPROLIFERATIVE CANCER STEM CELLS
Title (en)
ATP-BASED CELL SORTING AND HYPERPROLIFERATIVE CANCER STEM CELLS
Title (de)
ATP-BASIERTE ZELLSORTIERUNG UND HYPERPROLIFERATIVE KREBSSTAMMZELLEN
Title (fr)
TRI CELLULAIRE À BASE D'ATP ET CELLULES SOUCHES CANCÉREUSES HYPERPROLIFÉRANTES
Publication
Application
Priority
- US 201962900139 P 20190913
- IB 2020058524 W 20200914
Abstract (en)
[origin: WO2021048830A1] High mitochondrial ATP is a metabolic trait that confers hyper-proliferation, stemness, anchorage-independence, anti-oxidant capacity and multi-drug resistance in cancer cells. Under the present approach, intracellular ATP levels may be used as a metabolic biomarker to identify, separate, and purify an aggressive and hyper-proliferative cancer stem cell ("CSC") phenotype. Further, ATP may be combined with other CSC markers, e.g., CD44 or ALDH-activity, to beneficially fractionate the CSC population into sub-populations. For example, ATP-high/ CD44-high CSC sub-populations showed twice the level of anchorage-independent growth compared to ATP-low/CD44-high CSC sub-populations. Also disclosed are complementary bioinformatic data that implicate mitochondrial ATP synthesis in stemness, metastasis, and the detection of circulating tumor cells ("CTCs"), and a five-member, ATP-related metastasis gene-signature (ABCA2, ATP5F1C, COX20, NDUFA2 and UQCRB). The gene signature of the present approach may be used to identify CSCs having a dramatic increase in cell migration and invasion in vitro capacity, as well as spontaneous metastasis in vivo. The present approach also provides a cellular platform for systematically targeting stemness, multi-drug resistance, and metastasis in cancer cells.
IPC 8 full level
C12N 5/095 (2010.01); C12N 5/071 (2010.01); C12Q 1/6809 (2018.01); G01N 15/10 (2006.01); G01N 21/64 (2006.01); G01N 33/48 (2006.01); G01N 33/52 (2006.01); G01N 33/574 (2006.01)
CPC (source: EP IL KR US)
A61P 35/00 (2017.12 - EP IL); C12N 5/0693 (2013.01 - EP IL); C12N 5/0695 (2013.01 - EP IL KR); C12Q 1/008 (2013.01 - KR); C12Q 1/04 (2013.01 - KR); G01N 15/1459 (2013.01 - EP IL); G01N 33/5011 (2013.01 - EP IL US); G01N 33/5735 (2013.01 - EP IL KR US); G01N 33/57484 (2013.01 - EP IL KR US); G01N 2015/1006 (2013.01 - EP IL KR); G01N 2800/50 (2013.01 - EP IL KR)
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
WO 2021048830 A1 20210318; CA 3154301 A1 20210318; CN 114630901 A 20220614; EP 4028509 A1 20220720; EP 4028509 A4 20230823; IL 291306 A 20220501; JP 2022552458 A 20221216; KR 20220061190 A 20220512; US 2022365069 A1 20221117
DOCDB simple family (application)
IB 2020058524 W 20200914; CA 3154301 A 20200914; CN 202080074448 A 20200914; EP 20862338 A 20200914; IL 29130622 A 20220313; JP 2022516341 A 20200914; KR 20227011726 A 20200914; US 202017642935 A 20200914