EP 4047093 A1 20220824 - MRNA AND METHOD FOR PRODUCING SAME, AND APPARATUS FOR PRODUCING PROTEIN AND METHOD FOR PRODUCING PROTEIN
Title (en)
MRNA AND METHOD FOR PRODUCING SAME, AND APPARATUS FOR PRODUCING PROTEIN AND METHOD FOR PRODUCING PROTEIN
Title (de)
MRNA UND VERFAHREN ZU IHRER HERSTELLUNG SOWIE VORRICHTUNG ZUR HERSTELLUNG VON PROTEINEN UND VERFAHREN ZUR HERSTELLUNG VON PROTEINEN
Title (fr)
ARNM ET SON PROCÉDÉ DE PRODUCTION, ET APPAREIL DE PRODUCTION DE PROTÉINE ET PROCÉDÉ DE PRODUCTION DE PROTÉINE
Publication
Application
Priority
- JP 2019188411 A 20191015
- JP 2020121249 A 20200715
- JP 2020037706 W 20201005
Abstract (en)
Provided is an mRNA that is used for synthesis of a protein, including a translation region containing a start codon and a stop codon and an untranslated region positioned on the 5'-end side of the start codon, in which some of phosphate groups within the range of at least from the 5' end of the untranslated region to 15 nt on the 3'-end side of the start codon are substituted with phosphorothioate groups. Further provided is a method for producing an mRNA, including: a step of preparing a DNA template; a step of preparing an unmodified NTP containing ATP, GTP, CTP, and UTP, and a modified NTP in which at least one kind of phosphate group of the unmodified NTP is substituted with a phosphorothioate group; and a step of performing transcription reaction with RNA polymerase using the DNA template as a template and the unmodified NTP and the modified NTP as substrates.
IPC 8 full level
C12N 15/11 (2006.01); C12N 15/09 (2006.01); C12P 19/34 (2006.01); C12P 21/00 (2006.01)
CPC (source: EP US)
C12N 15/67 (2013.01 - EP US); C12P 19/34 (2013.01 - EP US); C12P 21/02 (2013.01 - EP US)
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
EP 4047093 A1 20220824; EP 4047093 A4 20230531; CN 114787350 A 20220722; JP WO2021075293 A1 20210422; US 2023193294 A1 20230622; WO 2021075293 A1 20210422
DOCDB simple family (application)
EP 20875862 A 20201005; CN 202080071801 A 20201005; JP 2020037706 W 20201005; JP 2021552323 A 20201005; US 202017768942 A 20201005