EP 4061386 A1 20220928 - A METHOD FOR PRODUCING MULTIVIRUS SPECIFIC T CELLS
Title (en)
A METHOD FOR PRODUCING MULTIVIRUS SPECIFIC T CELLS
Title (de)
VERFAHREN ZUR HERSTELLUNG VON MULTIVIRUSSPEZIFISCHEN T-ZELLEN
Title (fr)
PROCÉDÉ DE PRODUCTION DE CELLULES T SPÉCIFIQUES MULTIVIRUS
Publication
Application
Priority
- TR 201918044 A 20191119
- TR 2019051071 W 20191213
Abstract (en)
[origin: WO2021101466A1] The present invention relates to a method for producing multivirus specific T cells convenient for use for cellular therapy purposes against multiple viruses worldwide, comprising the steps of making donor selections using HLA tissue information data and apheresis of blood obtained from the donor; separating naive T cells (1) from apheresis allograft (2) by selectively labeling with magnetic CD45RA beads (3); performing CD45RA+ depletion with the help of a magnetic column (4) and collecting naive T cells (5) under the magnetic column (4); stimulating separated mononuclear T cells (5) with a viral peptide pool (6) at least once; feeding cytokines with IL-2 and IL-7 content (7) on different days to the T cell (8) population formed; multivirus specification of T cell (8) population by re-stimulation with viral peptide pool (6); ensuring the expansion of multivirus specific T cell (11) population by feeding cytokines (10) with IL-2, IL-7 and IL-15 content on different days; and determining the peptide-specific activity of the produced cells.
IPC 8 full level
A61K 35/17 (2015.01); C12N 5/00 (2006.01); G01N 33/50 (2006.01)
CPC (source: EP US)
A61K 39/12 (2013.01 - EP); A61K 39/4611 (2023.05 - EP US); A61K 39/464838 (2023.05 - EP US); C12N 5/0636 (2013.01 - EP US); A61K 2039/70 (2013.01 - EP); C12N 2710/10034 (2013.01 - EP); C12N 2710/16134 (2013.01 - EP); C12N 2710/16234 (2013.01 - EP); C12N 2710/22034 (2013.01 - EP); Y02A 50/30 (2018.01 - EP)
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
WO 2021101466 A1 20210527; EP 4061386 A1 20220928; EP 4061386 A4 20240110
DOCDB simple family (application)
TR 2019051071 W 20191213; EP 19928301 A 20191213