Global Patent Index - EP 4061386 A4

EP 4061386 A4 20240110 - A METHOD FOR PRODUCING MULTIVIRUS SPECIFIC T CELLS

Title (en)

A METHOD FOR PRODUCING MULTIVIRUS SPECIFIC T CELLS

Title (de)

VERFAHREN ZUR HERSTELLUNG VON MULTIVIRUSSPEZIFISCHEN T-ZELLEN

Title (fr)

PROCÉDÉ DE PRODUCTION DE CELLULES T SPÉCIFIQUES MULTIVIRUS

Publication

EP 4061386 A4 20240110 (EN)

Application

EP 19928301 A 20191213

Priority

  • TR 201918044 A 20191119
  • TR 2019051071 W 20191213

Abstract (en)

[origin: WO2021101466A1] The present invention relates to a method for producing multivirus specific T cells convenient for use for cellular therapy purposes against multiple viruses worldwide, comprising the steps of making donor selections using HLA tissue information data and apheresis of blood obtained from the donor; separating naive T cells (1) from apheresis allograft (2) by selectively labeling with magnetic CD45RA beads (3); performing CD45RA+ depletion with the help of a magnetic column (4) and collecting naive T cells (5) under the magnetic column (4); stimulating separated mononuclear T cells (5) with a viral peptide pool (6) at least once; feeding cytokines with IL-2 and IL-7 content (7) on different days to the T cell (8) population formed; multivirus specification of T cell (8) population by re-stimulation with viral peptide pool (6); ensuring the expansion of multivirus specific T cell (11) population by feeding cytokines (10) with IL-2, IL-7 and IL-15 content on different days; and determining the peptide-specific activity of the produced cells.

IPC 8 full level

A61K 35/17 (2015.01); C12N 5/00 (2006.01); G01N 33/50 (2006.01)

CPC (source: EP)

A61K 39/12 (2013.01); A61K 39/4611 (2023.05); A61K 39/464838 (2023.05); C12N 5/0636 (2013.01); A61K 2039/70 (2013.01); C12N 2710/10034 (2013.01); C12N 2710/16134 (2013.01); C12N 2710/16234 (2013.01); C12N 2710/22034 (2013.01); Y02A 50/30 (2018.01)

Citation (search report)

  • [A] WO 2018052947 A1 20180322 - BAYLOR COLLEGE MEDICINE [US]
  • [A] WO 2013119947 A1 20130815 - BAYLOR COLLEGE MEDICINE [US]
  • [A] VIJAYENDRA DASARI ET AL: "Prophylactic and therapeutic adenoviral vector-based multivirus-specific T-cell immunotherapy for transplant patients", MOLECULAR THERAPY- METHODS & CLINICAL DEVELOPMENT, vol. 3, 1 January 2016 (2016-01-01), GB, pages 1 - 9, XP055465882, ISSN: 2329-0501, DOI: 10.1038/mtm.2016.58
  • [A] P. J. HANLEY ET AL: "Functionally active virus-specific T cells that target CMV, adenovirus, and EBV can be expanded from naive T-cell populations in cord blood and will target a range of viral epitopes", BLOOD, vol. 114, no. 9, 27 August 2009 (2009-08-27), pages 1958 - 1967, XP055200362, ISSN: 0006-4971, DOI: 10.1182/blood-2009-03-213256
  • [A] ULRIKE GERDEMANN ET AL: "Rapidly Generated Multivirus-specific Cytotoxic T Lymphocytes for the Prophylaxis and Treatment of Viral Infections", MOLECULAR THERAPY, 17 July 2012 (2012-07-17), XP055034394, ISSN: 1525-0016, DOI: 10.1038/mt.2012.130
  • [A] OMER BILAL ET AL: "Administration of Most Closely HLA-Matched Multivirus-Specific T Cells for the Treatment of EBV, CMV, AdV, HHV6, and BKV Post Allogeneic Hematopoietic Stem Cell Transplant", BLOOD, AMERICAN SOCIETY OF HEMATOLOGY, US, vol. 126, no. 23, 3 December 2015 (2015-12-03), pages 622, XP086642640, ISSN: 0006-4971, DOI: 10.1182/BLOOD.V126.23.622.622
  • [A] NISHIYAMA-FUJITA YURIKO ET AL: "Generation of multivirus-specific T cells by a single stimulation of peripheral blood mononuclear cells with a peptide mixture using serum-free medium", CYTOTHERAPY, vol. 20, no. 9, 1 September 2018 (2018-09-01), GB, pages 1182 - 1190, XP093101449, ISSN: 1465-3249, DOI: 10.1016/j.jcyt.2018.05.009
  • See also references of WO 2021101466A1

Designated contracting state (EPC)

AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

DOCDB simple family (publication)

WO 2021101466 A1 20210527; EP 4061386 A1 20220928; EP 4061386 A4 20240110

DOCDB simple family (application)

TR 2019051071 W 20191213; EP 19928301 A 20191213