EP 4114931 A1 20230111 - METHODS FOR CELL FREE PROTEIN EXPRESSION OF MATURE POLYPEPTIDES DERIVED FROM ZYMOGENS AND PROPROTEINS
Title (en)
METHODS FOR CELL FREE PROTEIN EXPRESSION OF MATURE POLYPEPTIDES DERIVED FROM ZYMOGENS AND PROPROTEINS
Title (de)
VERFAHREN ZUR ZELLFREIEN PROTEINEXPRESSION VON REIFEN POLYPEPTIDEN AUS ZYMOGENEN UND PROPROTEINEN
Title (fr)
PROCÉDÉS POUR L'EXPRESSION DE PROTÉINES ACELLULAIRES DE POLYPEPTIDES MATURES DÉRIVÉS DE ZYMOGÈNES ET DE PROPROTÉINES
Publication
Application
Priority
- US 2020021211 W 20200305
- US 2020049226 W 20200903
Abstract (en)
[origin: WO2021178001A1] High throughput cell free protein synthesis methods and systems are provided for expression of active, mature enzymes and proteins derived from zymogens and proproteins. Zymogens and proproteins are expressed in their mature, active form in a cell free expression system, without cleavage of a pro-sequence to produce the active polypeptide.
IPC 8 full level
C12N 9/10 (2006.01); C12N 15/52 (2006.01)
CPC (source: EP KR)
A01N 25/10 (2013.01 - KR); A01N 25/28 (2013.01 - KR); A01N 63/50 (2020.01 - EP KR); A01P 1/00 (2021.08 - EP); A61K 8/66 (2013.01 - EP KR); A61K 8/736 (2013.01 - EP KR); A61K 9/1652 (2013.01 - EP KR); A61Q 19/00 (2013.01 - EP KR); C12N 9/1044 (2013.01 - EP KR); C12N 15/52 (2013.01 - EP); A61K 2800/10 (2013.01 - EP KR); A61K 2800/524 (2013.01 - EP KR); A61K 2800/57 (2013.01 - EP KR); C12Y 203/02013 (2013.01 - EP KR); Y02A 50/30 (2018.01 - EP)
C-Set (source: EP)
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
Designated validation state (EPC)
KH MA MD TN
DOCDB simple family (publication)
WO 2021178001 A1 20210910; EP 4114931 A1 20230111; EP 4114931 A4 20240501
DOCDB simple family (application)
US 2020049226 W 20200903; EP 20923242 A 20200903