EP 4121541 A2 20230125 - PRODUCTION OF SOLUBLE RECOMBINANT PROTEIN
Title (en)
PRODUCTION OF SOLUBLE RECOMBINANT PROTEIN
Title (de)
HERSTELLUNG EINES LÖSLICHEN REKOMBINANTEN PROTEINS
Title (fr)
PRODUCTION DE PROTÉINE RECOMBINANTE SOLUBLE
Publication
Application
Priority
- US 202016819775 A 20200316
- US 202062990083 P 20200316
- US 202163152954 P 20210224
- US 2021022126 W 20210312
Abstract (en)
[origin: WO2021188379A2] The invention is directed to methods and compositions for the expression and purification of products such as peptides and proteins in microorganisms. In particular, pre-products are expressed recombinantly, wherein the cytoplasm of the microorganism alters the expressed pre-products to produce products in an active/final or otherwise desirable form. Alterations associated with expression of a desired recombinant product include shifting of the redox state of the cytoplasm to allow proper protein folding, site-directed cleavage of pre-proteins to activate the protein, site-directed cleavage of an unwanted methionine from the N terminus of the protein, and/or one or more ligations to form desired protein configurations, all within the same cell.
IPC 8 full level
C12N 15/70 (2006.01); A61K 39/05 (2006.01); A61P 31/04 (2006.01); C07K 14/34 (2006.01); C12N 15/74 (2006.01); C12P 21/02 (2006.01)
CPC (source: EP KR)
C07K 1/16 (2013.01 - KR); C07K 14/005 (2013.01 - EP); C07K 14/31 (2013.01 - EP KR); C07K 14/33 (2013.01 - EP KR); C07K 14/34 (2013.01 - EP); C07K 14/5428 (2013.01 - EP KR); C12N 9/0051 (2013.01 - EP KR); C12N 9/485 (2013.01 - EP KR); C12N 15/70 (2013.01 - EP KR); C12P 21/02 (2013.01 - EP); C12P 21/06 (2013.01 - EP); C12Y 108/01007 (2013.01 - EP); C12Y 108/01008 (2013.01 - EP KR); C12Y 304/11018 (2013.01 - EP KR); C12N 2710/16222 (2013.01 - EP)
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
Designated validation state (EPC)
KH MA MD TN
DOCDB simple family (publication)
WO 2021188379 A2 20210923; WO 2021188379 A3 20211028; AU 2021239914 A1 20220908; CA 3168571 A1 20210923; CN 115867661 A 20230328; EP 4121541 A2 20230125; JP 2023517708 A 20230426; JP 7449000 B2 20240313; KR 20220154221 A 20221121
DOCDB simple family (application)
US 2021022126 W 20210312; AU 2021239914 A 20210312; CA 3168571 A 20210312; CN 202180021748 A 20210312; EP 21772422 A 20210312; JP 2022555779 A 20210312; KR 20227035931 A 20210312