EP 4130249 A4 20240508 - METHOD FOR OBTAINING ENDOMETRIAL MESENCHYMAL STEM CELLS FROM HUMAN MENSTRUAL BLOOD
Title (en)
METHOD FOR OBTAINING ENDOMETRIAL MESENCHYMAL STEM CELLS FROM HUMAN MENSTRUAL BLOOD
Title (de)
VERFAHREN ZUR GEWINNUNG ENDOMETRIALER MESENCHYMALER STAMMZELLEN AUS MENSCHLICHEM MENSTRUATIONSBLUT
Title (fr)
PROCÉDÉ D'OBTENTION DE CELLULES SOUCHES MÉSENCHYMATEUSES ENDOMÉTRIALES À PARTIR DE SANG MENSTRUEL HUMAIN
Publication
Application
Priority
- CN 202010259045 A 20200403
- CN 2021085202 W 20210402
Abstract (en)
[origin: EP4130249A1] Provided are a method for obtaining endometrial mesenchymal stem cells from human menstrual blood and the endometrial mesenchymal stem cells prepared thereby. The method include: 1) subjecting an aliquot of menstrual blood to cascade filtration through graded cell strainers of decreasing mesh sizes to separate tissue debris, wherein said cell strainers include a filter-through cell strainer for collecting filtrate and a retention cell strainer for collecting retentate, wherein the mesh size of the retention cell strainer is smaller than that of the filter-through cell strainer; 2) collecting the retentate on the retention cell strainer as the tissue debris; 3) culturing the tissue debris in a mesenchymal stem cells medium; and 4) collecting adherent cells as the endometrial mesenchymal stem cells.
IPC 8 full level
C12N 5/0775 (2010.01)
CPC (source: EP US)
C12N 5/0665 (2013.01 - EP US); C12Q 1/24 (2013.01 - US); C12N 2500/90 (2013.01 - US); C12N 2509/00 (2013.01 - US)
Citation (search report)
- [XA] SUN YULIANG ET AL: "High-yield isolation of menstrual blood derived endometrial stem cells by direct red blood cell lysis treatment", BIOLOGY OPEN, 1 January 2019 (2019-01-01), XP093144133, ISSN: 2046-6390, Retrieved from the Internet <URL:http://journals.logists.com/bio/bio/article-pdf/doi/10.1242/bio.038885/2051095/bio_038885v1.pdf> DOI: 10.1242/bio.038885
- [XA] LIU YANLI ET AL: "Biological characteristics of human menstrual blood-derived endometrial stem cells", JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, vol. 22, no. 3, 26 December 2017 (2017-12-26), RO, pages 1627 - 1639, XP093144351, ISSN: 1582-1838, Retrieved from the Internet <URL:https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjcmm.13437> DOI: 10.1111/jcmm.13437
- See references of WO 2021197459A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
EP 4130249 A1 20230208; EP 4130249 A4 20240508; CN 115516083 A 20221223; JP 2023519371 A 20230510; JP 7428823 B2 20240206; US 2023116841 A1 20230413; WO 2021197459 A1 20211007
DOCDB simple family (application)
EP 21778996 A 20210402; CN 2021085202 W 20210402; CN 202180025074 A 20210402; JP 2022558395 A 20210402; US 202117907318 A 20210402