Global Patent Index - EP 4144853 A1

EP 4144853 A1 20230308 - GENERATION OF HAPLOID PLANTS

Title (en)

GENERATION OF HAPLOID PLANTS

Title (de)

ERZEUGUNG VON HAPLOIDEN PFLANZEN

Title (fr)

GÉNÉRATION DE PLANTES HAPLOÏDES

Publication

EP 4144853 A1 20230308 (EN)

Application

EP 22181163 A 20151223

Priority

  • EP 14004389 A 20141223
  • EP 15817864 A 20151223
  • EP 2015081158 W 20151223

Abstract (en)

The present invention relates to non-transgenic and transgenic plants, preferably crop plants, having biological activity of a haploid inducer and comprising a polynucleotide which comprises a nucleotide sequence encoding a centromer histone H3 (CENH3) protein, wherein the polynucleotide comprises at least one mutation causing an alteration of the amino acid sequence of the CENH3 protein, and to a part of the part. Further, the invention provides methods of generating the inducer plants, methods of generating haploid and double haploid plants using the inducer plants as well as methods of facilitating cytoplasm exchange.

IPC 8 full level

C12N 15/82 (2006.01); A01H 1/08 (2006.01); A01H 5/06 (2018.01); A01H 5/10 (2018.01); A01H 6/20 (2018.01); C07K 14/415 (2006.01); C12N 15/29 (2006.01)

CPC (source: CN EA EP US)

A01H 1/021 (2021.01 - CN EA EP US); A01H 1/023 (2021.01 - CN EA EP US); A01H 1/08 (2013.01 - CN EA EP US); A01H 5/00 (2013.01 - EA); A01H 5/06 (2013.01 - CN EA EP US); A01H 5/10 (2013.01 - CN EA EP US); A01H 6/20 (2018.05 - CN EP US); C07K 14/415 (2013.01 - CN EA EP US); C12N 15/82 (2013.01 - EA); C12N 15/8287 (2013.01 - CN EP US); Y02A 40/146 (2018.01 - EP)

Citation (applicant)

  • RAVICHAN, NATURE, vol. 464, 2010, pages 615 - 618
  • ROST, BSANDER, C.: "Combining evolutionary information and neural networks to predict protein secondary structure", PROTEINS, vol. 19, no. 1, 1994, pages 55 - 72, XP000874041, DOI: 10.1002/prot.340190108
  • ROST, B.SANDER, C.: "Conservation and prediction of solvent accessibility in protein families", PROTEINS, vol. 20, no. 3, 1994, pages 216 - 26
  • ROST, B.CASADIO, R.FARISELLI, P.SANDER, C.: "Transmembrane helices predicted at 95% accuracy", PROTEIN SCI, vol. 4, no. 3, 1995, pages 521 - 33, XP008008201
  • NEUFFER, MG ET AL.: "Mutants of maize", 1997, COLD SPRING HARBOR LABORATORY

Citation (search report)

  • [XI] WO 2011044132 A1 20110414 - UNIV CALIFORNIA [US], et al
  • [XI] WO 2014110274 A2 20140717 - UNIV CALIFORNIA CORP [US], et al
  • [XDI] MARUTHACHALAM RAVI & SIMON W L CHAN: "Haploid plants produced by centromere-mediated genome elimination", NATURE, NATURE PUBLISHING GROUP, UNITED KINGDOM, vol. 464, no. 7288, 25 March 2010 (2010-03-25), pages 615 - 620, XP002677783, ISSN: 0028-0836, DOI: 10.1038/NATURE08842
  • [A] BRENDA MARIN-RODRIGUEZ: "Can Point Mutations in Kinetochore Proteins Create Haploid Plants in Arabidopsis thaliana?", UC DAVIS EXPLORATIONS, September 2014 (2014-09-01), pages 7 - 7, XP055191293, Retrieved from the Internet <URL:http://explorations.ucdavis.edu/docs/2014/2014explorations.pdf> [retrieved on 20150522]
  • [A] TAKAYOSHI ISHII ET AL: "Functional characterization of barley CENH3 variants", PLANT MOLECULAR CYTOGENETICS IN GENOMIC AND POSTGENOMIC ERA, September 2014 (2014-09-01), pages 51, XP055191331, Retrieved from the Internet <URL:http://real.mtak.hu/18302/1/abstract_book.pdf> [retrieved on 20150522]
  • [A] "Plant Centromere Biology", 8 April 2013 (2013-04-08), Oxford [u.a.], pages 67 - 82, XP055163252, ISBN: 978-1-119-94921-3, Retrieved from the Internet <URL:http://onlinelibrary.wiley.com/store/10.1002/9781118525715.ch6/asset/ch6.pdf?v=1&t=i55012yy&s=13933095b8df6c4f37caff5cd3f546a86de9d9f1> DOI: 10.1002/9781118525715.ch6
  • [A] M. RAVI ET AL: "The Rapidly Evolving Centromere-Specific Histone Has Stringent Functional Requirements in Arabidopsis thaliana", GENETICS, vol. 186, no. 2, 13 July 2010 (2010-07-13), pages 461 - 471, XP055142479, ISSN: 0016-6731, DOI: 10.1534/genetics.110.120337
  • [A] POOJA BHATNAGAR- MATHUR ET AL: "Engineering Centromeres for Haploidy Induction in Grain Legumes", ICRISAT ASIA REGIONAL PLANNING MEETING, PATANCHERU, INDIA, FEBRUARY 10-12, 2014, February 2014 (2014-02-01), Patancheru, India, XP055193834, Retrieved from the Internet <URL:http://ksiconnect.icrisat.org/wp-content/uploads/2014/02/6Pooja-DH-for-haploids.pdf> [retrieved on 20150605]
  • [A] IZABEL C. R. MORAES: "Structural requirements for CENH3 targeting to centromeric chromatin", PH. D. THESIS, February 2011 (2011-02-01), Halle (Saale), Germany, XP055191122, Retrieved from the Internet <URL:http://d-nb.info/1025136047/34> [retrieved on 20150522]
  • [A] MARUTHACHALAM RAVI ET AL: "A haploid genetics toolbox for Arabidopsis thaliana", NATURE COMMUNICATIONS, vol. 5, 31 October 2014 (2014-10-31), pages 5334, XP055191112, DOI: 10.1038/ncomms6334
  • [AP] RAHELEH KARIMI-ASHTIYANI ET AL: "Point mutation impairs centromeric CENH3 loading and induces haploid plants", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 112, no. 36, 20 August 2015 (2015-08-20), pages 11211 - 11216, XP055233810, ISSN: 0027-8424, DOI: 10.1073/pnas.1504333112

Designated contracting state (EPC)

AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

DOCDB simple family (publication)

EP 3037540 A1 20160629; AR 103226 A1 20170426; AU 2015370904 A1 20170608; AU 2015370904 B2 20210722; AU 2021225142 A1 20210930; AU 2021225142 B2 20230629; BR 112017013406 A2 20180206; BR 112017013406 B1 20240319; CA 2970123 A1 20160630; CL 2017001665 A1 20180126; CN 107205354 A 20170926; DK 3237623 T3 20220912; EA 039590 B1 20220214; EA 201791414 A1 20180228; EA 202193164 A1 20220301; EA 202193165 A1 20220301; EP 3237623 A2 20171101; EP 3237623 B1 20220713; EP 4141123 A1 20230301; EP 4144850 A1 20230308; EP 4144851 A1 20230308; EP 4144852 A1 20230308; EP 4144853 A1 20230308; EP 4144854 A1 20230308; EP 4144855 A1 20230308; ES 2926323 T3 20221025; HU E059654 T2 20221228; MX 2017008283 A 20171002; MX 2022015112 A 20230118; PL 3237623 T3 20221031; US 10844401 B2 20201124; US 2018139917 A1 20180524; US 2021079419 A1 20210318; WO 2016102665 A2 20160630; WO 2016102665 A3 20170316

DOCDB simple family (application)

EP 14004389 A 20141223; AR P150104235 A 20151221; AU 2015370904 A 20151223; AU 2021225142 A 20210830; BR 112017013406 A 20151223; CA 2970123 A 20151223; CL 2017001665 A 20170622; CN 201580075644 A 20151223; DK 15817864 T 20151223; EA 201791414 A 20151223; EA 202193164 A 20151223; EA 202193165 A 20151223; EP 15817864 A 20151223; EP 2015081158 W 20151223; EP 22181154 A 20151223; EP 22181156 A 20151223; EP 22181157 A 20151223; EP 22181163 A 20151223; EP 22181424 A 20151223; EP 22181430 A 20151223; EP 22181432 A 20151223; ES 15817864 T 20151223; HU E15817864 A 20151223; MX 2017008283 A 20151223; MX 2022015112 A 20170621; PL 15817864 T 20151223; US 201515539065 A 20151223; US 202017068487 A 20201012