EP 4153785 A1 20230329 - ISOTHERMAL REAL-TIME PCR METHOD FOR DETERMINING PRESENCE OF A PRE-DETERMINED RNA SEQUENCE IN A SAMPLE
Title (en)
ISOTHERMAL REAL-TIME PCR METHOD FOR DETERMINING PRESENCE OF A PRE-DETERMINED RNA SEQUENCE IN A SAMPLE
Title (de)
ISOTHERMES ECHTZEIT-PCR-VERFAHREN ZUR BESTIMMUNG DER ANWESENHEIT EINER VORBESTIMMTEN RNA-SEQUENZ IN EINER PROBE
Title (fr)
PROCÉDÉ DE PCR EN TEMPS RÉEL ISOTHERME POUR DÉTERMINER LA PRÉSENCE D'UNE SÉQUENCE D'ARN PRÉDÉTERMINÉE DANS UN ÉCHANTILLON
Publication
Application
Priority
- EP 20179109 A 20200609
- EP 2021065549 W 20210609
Abstract (en)
[origin: EP3922734A1] The present invention relates to a method for determining presence of a pre-determined RNA sequence in a sample, the method comprising the steps of adding one or more enzyme(s) providing activities of RNA- and DNA-dependent DNA polymerase activity and strand-displacement activity to the sample to be analysed for the presence of the pre-determined RNA sequence; simultaneously or subsequently adding at least five DNA primers to the sample to be analysed for the presence of the pre-determined RNA sequence, wherein at least one DNA primer comprises a sequence hybridisable to the RNA sequence and at least one DNA primer comprises a sequence hybridisable to the DNA sequence reverse-complementary to the RNA sequence; incubating the sample resulting from the previous steps at a fixed temperature; and determining whether an elongated double-stranded DNA sequence is present in the sample, wherein presence of the elongated double-stranded DNA sequence in the sample is indicative of the presence of the pre-determined RNA sequence in the sample wherein the pre-determined RNA sequence is part of an RNA virus.
IPC 8 full level
C12Q 1/6888 (2018.01)
CPC (source: EP)
C12Q 1/6888 (2013.01)
Citation (search report)
See references of WO 2021250140A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
Designated validation state (EPC)
KH MA MD TN
DOCDB simple family (publication)
EP 3922734 A1 20211215; AU 2021288610 A1 20230119; CA 3181838 A1 20211216; CN 116134156 A 20230516; EP 4153785 A1 20230329; JP 2023538180 A 20230907; KR 20230035317 A 20230313; WO 2021250140 A1 20211216
DOCDB simple family (application)
EP 20179109 A 20200609; AU 2021288610 A 20210609; CA 3181838 A 20210609; CN 202180056494 A 20210609; EP 2021065549 W 20210609; EP 21731167 A 20210609; JP 2022576084 A 20210609; KR 20237000746 A 20210609