EP 4175651 A1 20230510 - METHODS AND COMPOSITIONS FOR EDITING THE B2M LOCUS IN B CELLS
Title (en)
METHODS AND COMPOSITIONS FOR EDITING THE B2M LOCUS IN B CELLS
Title (de)
VERFAHREN UND ZUSAMMENSETZUNGEN ZUR BEARBEITUNG DES B2M-LOKUS IN B-ZELLEN
Title (fr)
PROCÉDÉS ET COMPOSITIONS DE MODIFICATION DU LOCUS B2M DANS DES CELLULES B
Publication
Application
Priority
- US 202063047978 P 20200703
- US 202063114131 P 20201116
- US 2021039953 W 20210630
Abstract (en)
[origin: WO2022006309A1] Some embodiments of the methods and compositions provided herein include preparing modified B cells. In some embodiments, an endogenous beta-2 microglobulin (B2M) gene in a B cell is modified. Some embodiments relate to increasing the resistance of modified B cells to killing by allogeneic immune cells. In some embodiments, the endogenous B2M gene is inactivated increasing the resistance of the modified B cell to killing by allogeneic immune cells. In some embodiments, a replacement MHC-I is inserted into an inactivated endogenous B2M gene increasing the resistance of the modified B cell to killing by allogeneic immune cells. Some embodiments include enriching for successfully modified cells.
IPC 8 full level
A61K 35/17 (2015.01); A61K 35/28 (2015.01); C12N 15/09 (2006.01); C12N 15/11 (2006.01)
CPC (source: EP KR US)
A61K 39/4612 (2023.05 - EP KR US); A61K 39/4621 (2023.05 - EP US); A61K 39/4622 (2023.05 - EP US); A61K 39/4631 (2023.05 - KR); A61K 39/4635 (2023.05 - KR); A61K 39/46434 (2023.05 - EP US); A61K 2239/26 (2023.05 - US); C07K 14/70539 (2013.01 - EP KR); C12N 9/22 (2013.01 - EP KR); C12N 15/102 (2013.01 - KR); C12N 15/1138 (2013.01 - EP KR); C12N 15/86 (2013.01 - KR); C12N 15/907 (2013.01 - EP KR); A61K 2121/00 (2013.01 - KR); A61K 2239/26 (2023.05 - EP); A61K 2300/00 (2013.01 - KR); C07K 2319/00 (2013.01 - EP KR); C07K 2319/60 (2013.01 - EP); C12N 2310/20 (2017.05 - EP KR); C12N 2750/14143 (2013.01 - EP KR)
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
Designated validation state (EPC)
KH MA MD TN
DOCDB simple family (publication)
WO 2022006309 A1 20220106; AU 2021300358 A1 20230202; BR 112022026534 A2 20230418; CA 3186620 A1 20220106; CN 116033910 A 20230428; EP 4175651 A1 20230510; EP 4175651 A4 20240814; IL 299491 A 20230201; JP 2023532917 A 20230801; KR 20230035048 A 20230310; MX 2022015788 A 20230227
DOCDB simple family (application)
US 2021039953 W 20210630; AU 2021300358 A 20210630; BR 112022026534 A 20210630; CA 3186620 A 20210630; CN 202180047549 A 20210630; EP 21832140 A 20210630; IL 29949122 A 20221225; JP 2022581341 A 20210630; KR 20237001885 A 20210630; MX 2022015788 A 20210630