EP 4179108 A1 20230517 - METHODS ANALYSING 5'-MONOPHOSPHORYLATED MRNA FRAGMENTS IN PROKARYOTIC CELLS
Title (en)
METHODS ANALYSING 5'-MONOPHOSPHORYLATED MRNA FRAGMENTS IN PROKARYOTIC CELLS
Title (de)
VERFAHREN ZUR ANALYSE VON 5'-MONOPHOSPHORYLIERTEN MRNA-FRAGMENTEN IN PROKARYOTISCHEN ZELLEN
Title (fr)
PROCÉDÉS D'ANALYSE DE FRAGMENTS D'ARNM 5'-MONOPHOSPHORYLÉS DANS DES CELLULES PROCARYOTES
Publication
Application
Priority
- GB 202010429 A 20200707
- EP 2021068185 W 20210701
Abstract (en)
[origin: GB2596822A] Methods of determining the identity of one or more messenger ribonucleic acid (mRNA) sequences being translated in a prokaryotic cell by determining the sequence of one or more 5’-monophosphorylated fragments of mRNA, wherein the cell is one in which 5’-3’ co-translational degradation of mRNA occurs such as may be evidenced by three nucleotide periodicity in the 5-m,onophosphorylated mRNA fragments. The invention also related to corresponding uses, kits of parts and libraries of sequences, and analysis of changes in the mRNA fragments in response to stimuli or a change in environmental conditions.
IPC 8 full level
C12Q 1/6806 (2018.01); C12Q 1/689 (2018.01)
CPC (source: EP GB)
C12Q 1/6806 (2013.01 - EP); C12Q 1/6869 (2013.01 - GB); C12Q 1/6888 (2013.01 - GB); C12Q 1/689 (2013.01 - EP)
Citation (search report)
See references of WO 2022008341A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
Designated validation state (EPC)
KH MA MD TN
DOCDB simple family (publication)
GB 202010429 D0 20200819; GB 2596822 A 20220112; CN 116096918 A 20230509; EP 4179108 A1 20230517; WO 2022008341 A1 20220113
DOCDB simple family (application)
GB 202010429 A 20200707; CN 202180054820 A 20210701; EP 2021068185 W 20210701; EP 21742068 A 20210701